Cell type-specific localization of human cardiac S1P receptors
Cell type-specific localization of human cardiac S1P receptors
复制标题
DOI：
10.1177/002215540205000507
复制
发表时间：
2002-05-01
影响因子：
3.2
通讯作者：
Calmels, T
中科院分区：
文献类型：
--
作者：
Mazurais, D;Robert, P;Calmels, T
1-磷酸鞘氨醇(S1P)来源于鞘磷脂的代谢，主要由生理和病理生理事件激活后的血小板合成、储存和释放。S1P通过内皮分化基因(EDG)家族的细胞表面G蛋白偶联受体，即EDG1、EDG3和EDG5作用于心血管组织。本研究的目的是评估EDG1、EDG3和EDG5受体在人类心血管组织中的精确分布，以探讨它们各自的生理意义。经Northern blotts检测，EDG1、EDG3和EDG5的表达水平由高到低依次为：EDG3主要在主动脉中表达。原位杂交(ISH)和免疫组织化学(IHC)的详细分析表明EDG1在心肌细胞和血管内皮细胞中有较强的表达。在心肌细胞中，EDG1受体可能与EDG3和EDG5共表达，尽管EDG1表现出最显著的表达模式。与EDG3和EDG5不同的是，EDG3和EDG5在人的主动脉中表达，但在主动脉中检测不到与EDG1表达相对应的信号。此外，EDG3在心脏血管的平滑肌细胞中也有表达。本研究结果为了解S1P受体在人类心血管组织中的表达模式提供了新的视角，表明这些受体在人类血管中的表达模式存在差异。
Sphingosine 1-phosphate (S1P), which derives from the metabolism of sphingomyelin, is mainly synthesized, stored, and released from platelets after activation by physiological and pathophysiological events. S1P acts in cardiovascular tissues through cell surface G-protein-coupled receptors of the endothelial differentiation gene (EDG) family, i.e., EDG1, EDG3 and EDG5. The aim of the present study was to assess the precise distribution of EDG1, EDG3, and EDG5 receptors expressed in human cardiovascular tissues to investigate their respective physiological implication. When assessed by Northern blots, EDG1, EDG3, and EDG5 displayed wide expression levels in decreasing order, respectively. In particular, EDG3 was mainly detected in the aorta. Detailed analysis by in situ hybridization (ISH) and immunohistochemistry (IHC) revealed strong EDG1 expression in cardiomyocytes and in endothelial cells of cardiac vessels. In cardiomyocytes, the EDG1 receptor is likely to be co-expressed with EDG3 and EDG5, although EDG1 exhibits the most prominent expression pattern. Unlike EDG3 and EDG5, which are expressed in the smooth muscle cell layer of the human aorta, no signal corresponding to EDG1 expression could be detected in the aorta. Moreover, only EDG3 expression was also found in smooth muscle cells of cardiac vessels. The present results provide new insight into the expression pattern of S1P receptors in human cardiovascular tissues, indicating a differential pattern of expression for these receptors in human vessels.