IDENTIFICATION OF GLYCOPROTEINS THAT ARE RECEPTORS FOR PEANUT AGGLUTININ ON IMMATURE (CORTICAL) MOUSE THYMOCYTES
IDENTIFICATION OF GLYCOPROTEINS THAT ARE RECEPTORS FOR PEANUT AGGLUTININ ON IMMATURE (CORTICAL) MOUSE THYMOCYTES
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DOI：
10.1016/0014-5793(86)80045-x
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发表时间：
1986-01-01
期刊：
影响因子：
3.5
通讯作者：
SHARON, N
中科院分区：
文献类型：
--
作者：
DEMAIO, A;LIS, H;SHARON, N
花生凝集素的结合被广泛用作未成熟小鼠胸腺细胞的标记物，并用于将这些细胞与成熟胸腺细胞分离。两个细胞表面糖蛋白，结合花生凝集素检测未分级以及未成熟的胸腺细胞凝集素覆盖和亲和层析：一个ofMr之间的170000和180000，和其他的，一个次要的组件，ofMr 110000，这两个都是部分唾液酸化。在成熟细胞上没有检测到花生凝集素受体，而去唾液酸化实验揭示了Mr110000糖蛋白的存在。这些发现证实了分离的胸腺细胞亚群的细胞表面糖蛋白标记在其碳水化合物部分的电泳分析。
Binding of peanut agglutinin is being widely used as a marker for immature mouse thymocytes and for the separation of these cells from the mature thymocytes. Two cell surface glycoproteins that bind peanut agglutinin were detected on unfractionated as well as immature thymocytes by lectin overlay and affinity chromatography: one ofMrbetween 170000 and 180000, and the other, a minor component, ofMr110000, both of which are partially sialylated. No receptors for peanut agglutinin were detected on the mature cells, whereas desialylation experiments revealed the presence of a glycoprotein ofMr110000. These findings were corroborated by electrophoretic analysis of cell surface glycoproteins of the isolated thymocyte subpopulations labeled in their carbohydrate moieties.