Immunolocalization of the thyrotropin-releasing hormone prohormone in the rat central nervous system.
Immunolocalization of the thyrotropin-releasing hormone prohormone in the rat central nervous system.
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DOI：
10.1210/endo-119-3-1210
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发表时间：
1986-09
期刊：
影响因子：
4.8
通讯作者：
R. Lechan;P. Wu;I. Jackson
中科院分区：
文献类型：
--
作者：
R. Lechan;P. Wu;I. Jackson
免疫反应性TRH激素原在大鼠中枢神经系统的分布进行了研究，免疫细胞化学使用的抗血清提出了对合成的十肽假设代表的哺乳动物TRH前体蛋白的一部分。反应产物在脑的几个区域中被鉴定，其分布典型地为先前针对三肽所描述的分布。然而，与TRH相反，免疫反应性促TRH主要局限于神经元胞体，很少见于轴突或轴突终末。此外，免疫反应性促甲状腺激素原存在于部分的端脑和脑干中，促甲状腺激素还没有被描述在神经元的免疫细胞化学。这些研究表明，在大脑的大多数区域，TRH激素原在细胞索马内被快速加工，而不是在轴突运输过程中，并提出了在大脑的某些区域，激素原的加工可能是非TRH肽的可能性，这可能具有生物学重要性。
The distribution of immunoreactive TRH prohormone in the rat central nervous system was studied by immunocytochemistry using an antiserum raised against a synthetic decapeptide hypothesized to represent a portion of the mammalian TRH precursor protein. Reaction product was identified in several regions of the brain in a distribution typical of that previously described for the tripeptide. In contrast to TRH, however, immunoreactive pro-TRH was largely confined to neuronal perikarya and only rarely seen in axons or axon terminals. In addition, immunoreactive pro-TRH was present in portions of the telencephalon and brainstem where TRH has not previously been described in neurons by immunocytochemistry. These studies indicate that in most regions of the brain the TRH prohormone is rapidly processed within the cell soma and not during axonal transport, and raise the possibility that in certain regions of the brain processing of the prohormone may be to non-TRH peptides, which may be of biological importance.