PHYSIOLOGICAL EXOPHORIA
PHYSIOLOGICAL EXOPHORIA
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DOI：
10.1111/j.1475-1313.1983.tb00613.x
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
PICKWELL, LD
中科院分区：
文献类型：
--
作者：
FREIER, BE;PICKWELL, LD
背景：口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中常见染色体臂5 p基因拷贝数增加，提示原癌基因激活，端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）是编码hTERT的候选基因。方法：采用组织芯片技术，分别对247例口腔鳞癌和105例喉、咽鳞状细胞癌（PSCC/LSCC）组织标本进行原位杂交（FISH）和免疫组化（IHC）检测，分析端粒酶基因拷贝数和hTERT蛋白表达。 OSCC中hTERT的阳性率（2.1%，4/191）低于PSCC/LSCC（9.9%，8/81）（P= 0.047）。 它可能是开发特异性抗肿瘤治疗方法的一个有前途的靶点。
Background:Gene copy number gain of chromosomal arm 5p is frequently found in oral squamous cell carcinoma (OSCC) suggesting the activation of proto‐oncogenes.TERTis a candidate gene encoding for human telomerase reverse transcriptase (hTERT). The aim of the present study was to elucidate the relevance ofTERTcopy number gain and high hTERT expression in OSCC.Methods:Fluorescencein situhybridization (FISH) forTERTand immunohistochemistry (IHC) for hTERT were performed to analyzeTERTcopy numbers and hTERT expression, respectively, on tissue microarray (TMA) sections includingn= 247 OSCC andn= 105 pharyngeal and laryngeal squamous cell carcinomas (PSCC/LSCC).Results:Increased hTERT protein expression was more frequently found in OSCC (71.1%, 155/218) than in PSCC/LSCC (36.0%, 35/89) (P< 0.001). By contrast, specificTERTamplifications were less common in OSCC (2.1%, 4/191) compared with PSCC/LSCC (9.9%, 8/81) (P= 0.047).Conclusions:High hTERT expression is a frequent finding in OSCC. It might be a promising target for the development of specific anti‐neoplastic therapy approaches.