Seizure Event Detection Using Intravital Two-Photon Calcium Imaging Data.
Seizure Event Detection Using Intravital Two-Photon Calcium Imaging Data.
复制标题
使用活体双光子钙成像数据检测癫痫事件。
DOI：
10.1101/2023.09.28.558338
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Berglund,Ken
中科院分区：
文献类型：
--
作者：
Stern,MatthewA;Cole,EricR;Gross,RobertE;Berglund,Ken
活体细胞钙成像已成为一种强大的工具，用于研究不同类型的神经元如何在微电路水平上相互作用以产生癫痫活动，并具有了解癫痫的新潜力。尽管在传统电生理学中存在许多方法来测量癫痫相关活动，但很少有钙成像的方法。目的演示使用钙成像检测癫痫相关事件的自动算法框架，包括检测峰前事件，癫痫波前的传播，以及群体水平活动和单个细胞的终端传播波。我们开发了一种算法，用于在癫痫相关事件期间精确检测群体和个体细胞的招募，该算法广泛地利用平均群体活动和高幅度斜率特征来检测单细胞的峰前峰和癫痫招募。我们将这种方法应用于戊四唑诱发的小鼠癫痫发作期间唤醒的体内双光子钙成像记录的数据。结果我们证明我们检测到的招募时间与专家审稿人提供的视觉识别标签一致，并且足够准确地模拟与癫痫发作相关的行波的时空进展。结论sour算法能够准确地检测细胞募集，为研究人员利用钙成像研究癫痫发作动态提供了有用的工具。
SignificanceIntravital cellular calcium imaging has emerged as a powerful tool to investigate how different types of neurons interact at the microcircuit level to produce seizure activity, with newfound potential to understand epilepsy. Although many methods exist to measure seizure-related activity in traditional electrophysiology, few yet exist for calcium imaging.AimTo demonstrate an automated algorithmic framework to detect seizure-related events using calcium imaging—including the detection of pre-ictal spike events, propagation of the seizure wavefront, and terminal spreading waves for both population-level activity and that of individual cells.ApproachWe developed an algorithm for precise recruitment detection of population and individual cells during seizure-associated events, which broadly leverages averaged population activity and high-magnitude slope features to detect single-cell pre-ictal spike and seizure recruitment. We applied this method to data recorded using awakein vivotwo-photon calcium imaging during pentylenetetrazol-induced seizures in mice.ResultsWe demonstrate that our detected recruitment times are concordant with visually identified labels provided by an expert reviewer and are sufficiently accurate to model the spatiotemporal progression of seizure-associated traveling waves.ConclusionsOur algorithm enables accurate cell recruitment detection and will serve as a useful tool for researchers investigating seizure dynamics using calcium imaging.