Block versus Random Amphiphilic Glycopolymer Nanopaticles as Glucose-Responsive Vehicles
Block versus Random Amphiphilic Glycopolymer Nanopaticles as Glucose-Responsive Vehicles
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块状与随机两亲性糖聚合物纳米颗粒作为葡萄糖响应载体
DOI：
10.1021/acs.biomac.5b01020
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发表时间：
2015-10-01
影响因子：
6.2
通讯作者：
Li, Chaoxing
中科院分区：
文献类型：
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作者：
Guo, Qianqian;Zhang, Tianqi;Li, Chaoxing
为了探索聚合物结构对其自组装聚集体的影响及其独特特性，本研究致力于通过 RAFT 聚合开发一系列两亲性嵌段和无规苯基硼酸基糖聚合物。两亲性糖聚合物在水溶液中成功自组装成具有窄尺寸分布的球形纳米粒子。对于具有相似单体组成的嵌段和无规共聚物，随着葡萄糖水平的增加，嵌段共聚物纳米颗粒表现出更规则的透射率变化，而无规共聚物纳米颗粒在不同葡萄糖介质中观察到更明显的尺寸变化和I/I-0行为更快的下降趋势。 MTT法检测的所有聚合物纳米粒子的细胞活力均高于80%，表明嵌段和无规共聚物均具有良好的细胞相容性。胰岛素可以被封装到两种纳米颗粒中，并且随机糖聚合物的胰岛素释放速率比块糖聚合物的胰岛素释放速率稍快。我们推测嵌段和无规糖聚合物之间的不同链构象在自组装纳米聚集体和潜在的葡萄糖敏感行为中发挥着重要作用。
To explore the effect of polymer structure on their self-assembled aggregates and their unique characteristics, this study was devoted to developing a series of amphiphilic block and random phenylboronic acid-based glycopolymers by RAFT polymerization. The amphiphilic glycopolymers were successfully self-assembled into spherically shaped nanoparticles with narrow size distribution in aqueous solution. For block and random copolymers with similar monomer compositions, block copolymer nanoparticles exhibited a more regular transmittance change with the increasing glucose level, while a more evident variation of size and quicker decreasing tendency in I/I-0 behavior in different glucose media were observed for random copolymer nanoparticles. Cell viability of all the polymer nanoparticles investigated by MTT assay was higher than 80%, indicating that both block and random copolymers had good cytocompatibility. Insulin could be encapsulated into both nanoparticles, and insulin release rate for random glycopolymer was slightly quicker than that for the block ones. We speculate that different chain conformations between block and random glycopolymers play an important role in self-assembled nanoaggregates and underlying glucose-sensitive behavior.