A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ
A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ
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DOI：
10.1002/gene.10030
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发表时间：
2002-03-01
期刊：
影响因子：
1.5
通讯作者：
Mann, JR
中科院分区：
文献类型：
--
作者：
Tang, SHE;Silva, FJ;Mann, JR
通过基因打靶将Cre重组酶表达盒插入到与129 S1/SvImJ（129 S1）等基因的小鼠胚胎干（ES）细胞系中的X连锁Hprt位点，然后通过ES细胞嵌合体将转基因导入129 S1小鼠。当该转基因的半合子雌性与携带loxP位点侧翼的新霉素选择盒的雄性交配时，无论Cre遗传如何，该盒总是被切除，并且没有可检测到的嵌合现象。这种“Cre-deleter”转基因系的有用性在于其效率和在小鼠品系和转基因位置方面的确定的遗传状态。创世纪32：199-202，2002。(C)2002 Wiley-Liss，Inc.
A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvImJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene. genesis 32: 199-202, 2002. (C) 2002 Wiley-Liss, Inc.