Graphic presentation and analysis of inhibition data from ligand-binding experiments.
Graphic presentation and analysis of inhibition data from ligand-binding experiments.
复制标题
配体结合实验的抑制数据的图形呈现和分析。
DOI：
10.1016/0003-2697(86)90306-4
复制
发表时间：
1986
影响因子：
2.9
通讯作者：
Bylund,DB
中科院分区：
文献类型：
--
作者：
Bylund,DB
一种用于表征受体亚型的技术涉及测量具有亚型选择性的激动剂或拮抗剂对不具有亚型选择性的放射性配基结合的抑制。虽然这样的数据通常是使用计算机程序计算的，但数据的图形表示通常是有用的。到目前为止，使用的是形式为P=−(Pi)(IC50)+1(其中P是抑制百分率，I是缓蚀剂浓度)的“改进的Scatchard”或“Hofstee”图。我们描述了形式B=−(B·i)(1/IC_(50))+B_0(其中B是结合的放射性配基的浓度，B0是在没有抑制剂的情况下结合的放射性配基的浓度)的交替曲线图。这种方法的重要优点是，数据不需要以百分比抑制来计算，这消除了将实验确定的B0值包含在其他值中所涉及的误差。
One technique for the characterization of receptor subtypes involves measuring the inhibition of the binding of a radioligand which is not subtype selective by agonists or antagonists which are subtype selective. Although such data are routinely calculated using computer programs, it is often useful to have a graphical representation of the data. Until now, a “modified Scatchard” or “Hofstee” plot of the form P = −( P I )( IC50) + 1 (where P is percentage inhibition and I is the inhibitor concentration) has been used. We describe an alternate plot of the form B = −(B·I)(1/IC50) + B0(where B is the concentration of bound radioligand and B0is the concentration of radioligand bound in the absence of the inhibitor). This method has the important advantage that the data need not be calculated as percentage inhibition which eliminates the error involved in the experimentally determined B0value being included in the other values.