EXPRESSION OF SIGMA RECEPTOR 1 mRNA AND PROTEIN IN RAT RETINA
EXPRESSION OF SIGMA RECEPTOR 1 mRNA AND PROTEIN IN RAT RETINA
复制标题
大鼠视网膜中 Sigma 受体 1 mRNA 和蛋白的表达
DOI：
10.1016/j.neuroscience.2010.03.006
复制
发表时间：
2010-06-02
期刊：
影响因子：
3.3
通讯作者：
Yang, X. L.
中科院分区：
文献类型：
--
作者：
Liu, L. L.;Wang, L.;Yang, X. L.
σ受体（sigma receptor，sigma R）是一种独特的非阿片类、非苯环利定类脑受体，可与多种精神药物、神经甾体和其他合成化合物如（+）喷他佐辛等结合。在这项工作中，我们研究了sigma R1在大鼠视网膜的表达，通过逆转录-聚合酶链反应（RT-PCR）分析和免疫荧光双标记。RT-PCR分析显示大鼠视网膜中存在sigma R1 mRNA。此外，标记为西格玛R1弥漫分布在外和内丛状层。在内核层和神经节细胞层的许多细胞中也观察到sigma R1免疫反应性（IR）。在视网膜外层，钙结合蛋白标记的所有水平细胞中均表达sigma R1。在蛋白激酶C（PKC）、recoverin和超极化激活的环核苷酸门控钾通道4（HCN 4）标记的双极细胞中，未检测到sigma R1-IR，包括视杆细胞为主的ON型双极细胞、2型、3型、5型和8型双极细胞。在视网膜内层，大多数GABA能无长突细胞，包括多巴胺能和胆碱能细胞，分别被酪氨酸羟化酶（TH）和胆碱乙酰转移酶（ChAT）染色，表达sigma R1。一些甘氨酸能无长突细胞也被sigma R1标记，但甘氨酸能无长突细胞没有被标记。荧光金逆行标记的神经节细胞胞体几乎全部可见sigma R1-IR。这些结果表明，sigma R1可能在视网膜中具有神经调节和神经保护作用。(C)2010年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Sigma receptor (sigma R), known as a unique nonopiate, nonphencyclidine brain receptor, can bind diverse classes of psychotropic drugs, neurosteroids and other synthetic compounds, such as (+)pentazocine, etc. Two types of sigma Rs have been identified: sigma R1 and sigma R2. In this work, we examined the expression of sigma R1 in rat retina by reverse transcription-polymerase chain reactive (RT-PCR) analysis and immunofluorescence double labeling. RT-PCR analysis showed that sigma R1 mRNA was present in rat retina. Furthermore, labeling for sigma R1 was diffusely distributed in the outer and inner plexiform layers. The sigma R1-immunoreactivity (IR) was also observed in many cells in the inner nuclear layer and the ganglion cell layer. In the outer retina sigma R1 was expressed in all horizontal cells labeled by calbindin. In contrast, no sigma R1-IR was detected in several subtypes of bipolar cells, including rod-dominant ON-type bipolar cells, types 2, 3, 5 and 8 bipolar cells, labeled by protein kinase C (PKC), recoverin and hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4) respectively. In the inner retina, most of GABAergic amacrine cells, including dopaminergic and cholinergic ones, stained by tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) respectively, expressed sigma R1. Some glycinergic amacrine cells were also labeled by sigma R1, but glycinergic All amacrine cells were not labeled. In addition, sigma R1-IR was seen in almost all somata of the ganglion cells retrogradely labeled by fluorogold. These results suggest that sigma R1 may have neuromodulatory and neuroprotective roles in the retina. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.