GROWTH-CHARACTERISTICS OF ATYPICAL MYCOBACTERIA IN WATER AND THEIR COMPARATIVE RESISTANCE TO DISINFECTANTS
GROWTH-CHARACTERISTICS OF ATYPICAL MYCOBACTERIA IN WATER AND THEIR COMPARATIVE RESISTANCE TO DISINFECTANTS
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DOI：
10.1128/aem.36.6.839-846.1978
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发表时间：
1978-01-01
影响因子：
4.4
通讯作者：
AGUERO, SM
中科院分区：
文献类型：
--
作者：
CARSON, LA;PETERSEN, NJ;AGUERO, SM
随着IV组非典型分枝杆菌作为各种[人类]感染的病原体的重要性日益增加，进行了研究以确定它们在水中的生长能力以及它们对用于净化医院设备的消毒剂的相对抗性。从患者的腹膜液和腹膜透析机分离的龟分枝杆菌（TM菌株）能够在商业蒸馏水中繁殖，在25 ℃下世代时间。C范围为8-15 h。达到105-106个细胞/ml的水平，并且这些稳定期群体在1年期间仅略微下降。TM株M.在商业蒸馏水中培养的龟在2%甲醛水溶液（HCHO）中显示存活长达24小时;在8%HCHO中，在2小时取样期内观察到存活计数仅减少2个对数。参考ATCC菌株M. chelonei和M. 2%HCHO暴露2 h或8%HCHO暴露15 min后，偶然性细胞迅速失活，无存活细胞。在2%碱性戊二醛中，TM菌株存活60 min，而ATCC菌株在接触2 min后无存活者。所有的M。chelonei和M.偶然菌株在暴露于0.3和0.7 μ g游离Cl/ml浓度、pH 7下60分钟后存活。
With the increasing significance of group IV atypical mycobacteria as etiological agents in a variety of [human] infections, studies were conducted to determine their growth capabilities in water and their comparative resistance to disinfectants used to decontaminate hospital equipment. Isolates of Mycobaterium chelonei (TM strains) from peritoneal fluids of patients and peritoneal dialysis machines were able to multiply in commercial distilled water, with generation times at 25.degree. C ranging from 8-15 h. Levels of 105-106 cells/ml were attained, and these stationary-phase populations declined only slightly over a 1 yr period. TM strains of M. chelonei cultured in commercial distilled water showed survivors in 2% aqueous formaldehyde (HCHO) solutions up to 24 h; in 8% HCHO, only a 2-log reduction in viable counts was observed over a 2-h sampling period. Reference ATCC strains of M. chelonei and M. fortuitum wer rapidly inactivated, with no survivors after 2 h of exposure to 2% HCHO or 15 min of exposure to 8% HCHO. In 2% alkaline glutaraldehyde, TM strains survived 60 min, whereas ATCC strains showed no survivors after 2 min of contact time. All M. chelonei and M. fortuitum strains survived 60 min of exposure to concentrations of 0.3 and 0.7 .mu.g of free Cl/ml at pH 7.