Enhanced production of interleukin-6 (IL-6), oncostatin M and soluble IL-6 receptor by cultured peripheral blood mononuclear cells from patients with systemic sclerosis
Enhanced production of interleukin-6 (IL-6), oncostatin M and soluble IL-6 receptor by cultured peripheral blood mononuclear cells from patients with systemic sclerosis
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DOI：
10.1093/rheumatology/38.7.612
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发表时间：
1999-07-01
期刊：
影响因子：
5.5
通讯作者：
Takehara, K
中科院分区：
文献类型：
--
作者：
Hasegawa, M;Sato, S;Takehara, K
目标。目的：探讨系统性硬化症（SSc）患者外周血单个核细胞（PBMC）自发产生白细胞介素6 （IL-6）、肿瘤抑制素M （OSM）、可溶性IL-6受体（sIL-6R）和可溶性gp130 （sgp130）是否增加。采用酶联免疫吸附法检测SSc患者（33例）和健康对照（20例）的PBMC培养上清。SSc患者IL-6、OSM和sIL-6R的产生水平明显高于对照组。然而，与对照组相比，SSc患者上清液中的sgp130水平没有显著升高。可溶性IL-6R水平与ssc患者肺纤维化严重程度显著相关。PBMC中IL-6、OSM和sIL-6R的增加可能共同参与了SSc的发病过程。特别是，PBMC产生的sIL-6R的增强可能通过增强SSc中IL-6信号转导与肺纤维化的发展有关，因为sIL-6R可以作为IL-6激动剂。
Objectives. To determine whether the spontaneous production of interleukin 6 (IL-6), oncostatin M (OSM), soluble IL-6 receptor (sIL-6R) and soluble gp130 (sgp130) from peripheral blood mononuclear cells (PBMC) is increased in patients with systemic sclerosis (SSc).Methods. The culture supernatants of PBMC from patients with SSc (n = 33) and healthy controls (n = 20) were examined by enzyme-linked immunosorbent assay.Results. The production levels of IL-6, OSM and sIL-6R were significantly higher in patients with SSc than in controls. However, sgp130 levels in supernatants from patients with SSc were not significantly elevated when compared with those from controls. Soluble IL-6R levels correlated significantly with the severity of pulmonary fibrosis in patients with SSc.Conclusions. The enhanced production of IL-6, OSM and sIL-6R from PBMC may cooperatively contribute to the disease process in SSc. In particular, enhanced sIL-6R production from PBMC may be related to the development of pulmonary fibrosis via enhancement of IL-6 signal transduction in SSc, since sIL-6R can act as an agonist of IL-6.