After All, Only Millions?

After All, Only Millions?
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DOI:
10.1128/mbio.00999-16
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发表时间:
2016-07-05
期刊:
影响因子:
6.4
通讯作者:
Rosselló-Móra R
Rosselló-Móra R
中科院分区:
生物学1区
文献类型:
--
作者:
Amann R;Rosselló-Móra R

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最近在mBio(P. D.)上发表的关于细菌和细菌物种普查的最新情况。施洛斯河A.吉拉德,T. Martin,J. Edwards和J. C. Thrash,mBio 7:e00201-16,2016,http://dx. doi. org/10. 1128/mBio.00201 - 16)再次表明,尽管测序工作不断增加,但16 S rRNA基因的基于PCR的检索接近饱和。平均而言,今天分析的基因中有95%与公共数据库中的基因相同,稀疏分析表明大约三分之一的细菌和古细菌多样性已经被覆盖。因此,尽管估计有多达1012种微生物物种,但应该考虑这样的选择,即地球上的细菌和细菌普查可能最终只产生数百万种物种。
An update on the census of species of Archaea and Bacteria published recently in mBio (P. D. Schloss, R. A. Girard, T. Martin, J. Edwards, and J. C. Thrash, mBio 7:e00201-16, 2016, http://dx.doi.org/10.1128/mBio.00201-16) showed again that, despite ever-increasing sequencing efforts, the PCR-based retrieval of 16S rRNA genes is approaching saturation. On average, 95% of the genes analyzed today are identical to those present in public databases, with rarefaction analysis indicating that about one-third of the bacterial and archaeal diversity has already been covered. Therefore, despite estimates of up to 1012 microbial species, the option should be considered that the census of Archaea and Bacteria on planet Earth might yield only millions of species after all.