Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27
Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27
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DOI：
10.1006/geno.1996.4544
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发表时间：
1997-02-15
期刊：
影响因子：
4.4
通讯作者：
Farber, JM
中科院分区：
文献类型：
--
作者：
Liao, F;Lee, HH;Farber, JM
根据趋化因子受体的保守序列，利用聚合酶链式反应，我们克隆了人类G蛋白偶联受体基因家族的一个成员。我们将这个新基因命名为STRL22，它被预测为编码与趋化因子受体相关的受体，包括IL8RA(CXCR1)、IL8RB(CXCR2)和CXCR4/Fusin，以及孤儿受体EBI1和BLR1。与白细胞生物学中的作用一致，STRL22在淋巴细胞和淋巴组织中表达。我们已将STRL22定位在染色体6q27上。STRL22的cDNAs反映了5‘-非翻译区的替代(或不完全)的mRNA处理，这一现象在分析其他趋化受体基因时发现。与大多数趋化因子受体基因不同，STRL22编码序列不限于单个外显子。(C)1997年学术出版社。
Using PCR with pools of primers based on conserved sequences in chemokine receptors, we have cloned a human member of the G-protein-coupled receptor gene family. The new gene, which we have named STRL22, is predicted to encode a receptor related to chemokine receptors, including IL8RA (CXCR1), IL8RB (CXCR2), and CXCR4/fusin, and to the orphan receptors EBI1 and BLR1. Consistent with a role in leukocyte biology, STRL22 is expressed in lymphocytes and in lymphoid tissue. We have mapped STRL22 to chromosome 6q27. STRL22 cDNAs reflect alternative (or incomplete) mRNA processing in the 5'-nontranslated region, a phenomenon found in analysis of other chemoattractant receptor genes. In contrast to most chemokine receptor genes, the STRL22 coding sequence is not limited to a Single exon. (C) 1997 Academic Press.