Detoxification of aflatoxin B1 by Stenotrophomonas sp. CW117 and characterization the thermophilic degradation process
Detoxification of aflatoxin B1 by Stenotrophomonas sp. CW117 and characterization the thermophilic degradation process
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寡养单胞菌对黄曲霉毒素 B1 的解毒作用
DOI：
10.1016/j.envpol.2020.114178
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发表时间：
2020-06-01
影响因子：
8.9
通讯作者：
Zhou, Yu
中科院分区：
文献类型：
--
作者：
Cai, Mengyu;Qian, Yingying;Zhou, Yu
真菌毒素是一种高毒性、广泛分布于食品中的污染物。本研究以黄曲霉毒素B1（AFB 1）降解菌S.对嗜酸胺菌CW 117的脱毒特性进行了研究。CW 117对45 μ g/L的底物AFB_1在24 h内基本降解，对4.1 mg/L的底物AFB_1在48 h内基本降解。降解24 h后，脱毒培养物的生物毒性被消除。CW 117菌株对黄曲霉毒素B_1（特别是低浓度黄曲霉毒素B_1）的高效降解表明其对食品和饲料的脱毒开发具有潜在的意义。无细胞上清液中存在的活性降解组分。随着培养温度的升高（0-90 ℃），降解率不断增加，甚至在90 ℃时保持稳定。降解是最佳的pH值为6-7，只有部分抑制金属螯合剂（EDTA和EGTA），蛋白酶K，和蛋白质变性剂（十二烷基硫酸钠，SDS）。CW 117重组漆酶rLC 1（0.5mg/mL）在24 h内对AFB 1的降解率为29.3%，而无细胞培养上清在相同时间内对毒素的降解率为76.7%，蛋白质含量明显降低。结果表明，CW 117通过酶和小分子氧化物的组合降解AFB 1。(C)2020爱思唯尔有限公司版权所有。
Mycotoxins are high toxic, widely distributed contaminants in foodstuff. In this study, a aflatoxin B1 (AFB1) degrading strain S. acidoaminiphila CW117 was screened, and its detoxification characteristics were investigated. Substrate AFB1 at 45 mu g/L was degraded by CW117 within 24 h; meanwhile, 4.1 mg/L AFB1 was almost degraded within 48 h. After 24 h degradation, the biotoxicity of the detoxified culture was eliminated. Strain CW117 efficient degradation to AFB1 (especially to low AFB1 concentrations) suggested its potential significance to detoxification development on food and feedstuff. The active degradation components present in the cell-free supernatant. The degradation ratio increased constantly with increasing incubation temperature raised (0-90 degrees C) and was even stable at 90 degrees C. Degradation was optimal at pH 6-7, and was only partially inhibited by metal-chelators (EDTA and EGTA), proteinase K, and a protein denaturant (sodium dodecyl sulfate, SDS). The recombinant laccase rLC1 (0.5 mg/mL) from CW117 degraded 29.3% of AFB1 within 24 h; however, the cell-free supernatant degraded 76.7% of the toxin in same time, with much lower protein content. The results indicated the CW117 degrades AFB1 via a combination of enzymes and micro-molecule oxides. (C) 2020 Elsevier Ltd. All rights reserved.