A 2ND THROMBOSPONDIN GENE IN THE MOUSE IS SIMILAR IN ORGANIZATION TO THROMBOSPONDIN-1 BUT DOES NOT RESPOND TO SERUM
A 2ND THROMBOSPONDIN GENE IN THE MOUSE IS SIMILAR IN ORGANIZATION TO THROMBOSPONDIN-1 BUT DOES NOT RESPOND TO SERUM
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DOI：
10.1073/pnas.88.19.8636
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
FRAMSON, P
中科院分区：
文献类型：
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作者：
BORNSTEIN, P;DEVARAYALU, S;FRAMSON, P
第二个表达的凝血酶反应蛋白(TSP)基因Thbs2已经在小鼠中被鉴定出来。与Thbs1相比，Thbs2外显子/内含子组织高度保守，外显子大小和内含子中断阅读框的模式被保留，但在编码序列上有明显的差异，主要是在前7个外显子。另一方面，两个TSP中编码I、II和III型重复序列的外显子的DNA和翻译的氨基酸序列要保守得多。Thbs2基因位于17号染色体A3带，而Thbs1基因位于2号染色体F带。与Thbs1基因不同的是，Thbs2基因在NIH3T3细胞中不受血清诱导，两个基因的启动子序列也有很大差异。因此，这两个TSP很可能执行相关但不同的功能。
A second, expressed thrombospondin (TSP) gene, Thbs2, has been identified in the mouse. The exon/intron organization of Thbs2 is highly conserved in comparison with Thbs1 in that exon size and the pattern of interruption of the reading frame by introns are preserved, but there is a marked divergence in coding sequence, primarily in the first 7 exons. On the other hand, the DNA and translated amino acid sequences of exons coding for the type I, II, and III repeats in the two TSPs are far better conserved. Thbs2 is located on chromosome 17, band A3, whereas Thbs1 was found on chromosome 2, band F. In marked contrast to Thbs1, the Thbs2 gene is not induced by serum in NIH 3T3 cells; promoter sequences in the two genes are also very different. It is therefore likely that the two TSPs perform related but distinct functions.