Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis)
Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis)
复制标题
DOI：
10.1034/j.1600-0684.2001.300205.x
复制
发表时间：
2001-04-01
影响因子：
0.7
通讯作者：
Hayes, ES
中科院分区：
文献类型：
--
作者：
Feradis, AH;Pawitri, D;Hayes, ES
本文研究了食蟹猴(Macaca Fuscularis)附睾针活检精子的活力、形态和冷冻保存。在采集时，附睾精子(23×10(6)+/-4×10(6)精子/样本；611×10(6)+/-116×10(6)精子/毫升；n=18)活的(79+/-2%)，活动的(67+/-2%)，显示完整的膜(65+/-2%)。在处理介质中保持在室温下的精子，随着时间的推移，精子的活力降低，但头对头的凝集作用有限。含有6%甘油和二甲基亚砜(DMSO)的Tris蛋黄稀释剂对功能形态没有显著影响，而含有丙二醇的蛋黄稀释剂显著降低了活力、存活率和膜的完整性。冷冻储存降低了与冷冻保护剂无关的所有功能形态的测量。甘油和DMSO的解冻后运动能力优于丙二醇。甘油浓度的变化(4%、6%和8%)对解冻后精子的功能形态产生了模棱两可的影响。对于实验室和现场采集非人类灵长类动物的精子来说，针刺活检可能是一种有用的技术。
We have examined the motility, morphology, and cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fuscicularis). At collection, epididymal sperm (23 x 10(6) +/- 4 x 10(6) sperm/sample; 611 x 10(6) +/- 116 x 10(6) sperm/ml; n = 18) were alive (79 +/- 2%), motile (67 +/- 2%), and exhibited intact membranes (65 +/- 2%). Sperm maintained at room temperature in handling medium exhibited decreased motility over time, but head-to-head agglutination was limited. Tris egg-yolk extender containing 6% glycerol and dimethylsulfoxide (DMSO) did not significantly affect functional morphology, whereas extender containing propanediol significantly reduced motility, survival, and membrane integrity. Cryostorage reduced all measures of functional morphology independent of cryoprotectant. Post-thaw motility was superior for glycerol and DMSO compared to propanediol. Variation in glycerol concentration (4, 6, and 8%) produced equivocal effects on sperm functional morphology post-thaw. Needle biopsy may be a useful technique for laboratory and field-based collection of spermatozoa from nonhuman primates.