Radiosynthesis and Biological Distribution of 18F-Labeled Perfluorinated Alkyl Substances
Radiosynthesis and Biological Distribution of 18F-Labeled Perfluorinated Alkyl Substances
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DOI：
10.1021/acs.estlett.7b00042
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发表时间：
2017-06-01
影响因子：
10.9
通讯作者：
Lapi, Suzanne E.
中科院分区：
文献类型：
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作者：
Burkemper, Jennifer L.;Aweda, Tolulope A.;Lapi, Suzanne E.
建立了一种新的全氟烷基物质(PFAS)18氟标记方法，并对纯化后的PFAS在健康小鼠体内的稳定性和生物分布进行了评价。[F-18]PFOA(C8)、[F-18]PFHxA(C6)和[F-18]PFBA(C4)三种全氟辛酸很容易被标记和分离，平均产率在12%到31%之间。每种化合物在0.1%氢氧化铵(NH4OH)、甲醇、生理盐水以及人、小鼠和大鼠血清中的稳定性被监测。完整的、放射性标记的PFAS的量通过放射性瞬时薄层色谱测定，并通过随时间观察到的游离氟18的量来计算。所有化合物在0.1%NH4OH甲醇和生理盐水中高度稳定，
A novel method for radiolabeling perfluorinated alkyl substances (PFAS) with fluorine 18 has been developed, and after purification, the stability and biological distribution in healthy mice were evaluated. Three PFAS, [F-18]PFOA (C8), [F-18]PFHxA (C6), and [F-18]PFBA (C4), were readily labeled and isolated in average yields between 12 and 31%. The stability of each compound was monitored in 0.1% ammonium hydroxide (NH4OH) in methanol, in saline, and in human, mouse, and rat sera. The amount of intact, radiolabeled PFAS was determined by radiometric instant thin layer chromatography and was calculated by the amount of free fluorine 18 observed over time. All compounds were highly stable in 0.1% NH4OH in methanol and saline, with