THE PHYLOGENY OF THE GENUS CLOSTRIDIUM - PROPOSAL OF 5 NEW GENERA AND 11 NEW SPECIES COMBINATIONS
THE PHYLOGENY OF THE GENUS CLOSTRIDIUM - PROPOSAL OF 5 NEW GENERA AND 11 NEW SPECIES COMBINATIONS
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DOI：
10.1099/00207713-44-4-812
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发表时间：
1994-10-01
期刊：
影响因子：
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通讯作者：
FARROW, JAE
中科院分区：
文献类型：
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作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通过PCR直接测序确定了34个命名和未命名的梭菌菌株的16 S rRNA基因序列，并与80多个先前确定的梭菌序列和先前公布的其他低G+ C含量革兰氏阳性菌属的代表性种的序列进行了比较，从而提供了梭菌的系谱相互关系的几乎完整的图片。我们的系统发育分析的结果证实和扩展以前的研究结果表明，属梭菌是非常异质的，与许多物种在遗传上与其他孢子形成和非孢子形成属混合。梭菌属显然需要进行重大修订，在本研究和以前的研究中定义的rRNA结构可能为未来的分类学重组提供良好的基础。重组的问题和不同的可能性进行了讨论，在光的表型和系统发育数据，并提出了一个可能的层级结构的梭菌及其近亲。根据表型标准和系统发育分析的结果，提出了5个新属和11个新组合：Caloramator gen. nov.，与Caloramator fervidus comb. nov.; Filifactor gen. nov.，与Filifactor villosus comb. nov.;穆雷拉根，与Moorella thermoacetica comb. nov.和Moorella thermoautotrophica comb. nov.; Oxygen. nov.，与Oxyphilpfenniophilcomb. nov.;草食虫属，与Oxalophagus peculicus comb. nov.;巴氏真杆菌;硬粒类芽孢杆菌;基武热空气动力学新组合Thermoanaerobium thermocopriae comb，nov.和热厌氧杆菌Thermoanerobacterium thermosaccharolyticum comb. nov.
The 16S rRNA gene sequences of 34 named and unnamed clostridial strains were determined by PCR direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- G+C-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of the clostridia. The results of our phylogenetic analysis corroborate and extend previous findings in showing that the genus Clostridium is extremely heterogeneous, with many species phylogenetically intermixed with other spore-forming and non-spore-forming genera. The genus Clostridium is clearly in need of major revision, and the rRNA structures defined in this and previous studies may provide a sound basis for future taxonomic restructuring. The problems and different possibilities for restructuring are discussed in light of the phenotypic and phylogenetic data, and a possible hierarchical structure for the clostridia and their close relatives is presented. On the basis of phenotypic criteria and the results of phylogenetic analyses the following five new genera and 11 new combinations are proposed: Caloramator gen. nov., with Caloramator fervidus comb. nov.; Filifactor gen. nov., with Filifactor villosus comb. nov.; Moorella gen, nov., with Moorella thermoacetica comb. nov. and Moorella thermoautotrophica comb. nov.; Oxobacter gen. nov., with Oxobacter pfennigii comb. nov.; Oxalophagus gen. nov., with Oxalophagus oxalicus comb. nov.; Eubacterium barkeri comb. nov.; Paenibacillus durum comb. nov.; Thermoanaerobacter kivui comb. nov.; Thermoanaerobacter thermocopriae comb, nov.; and Thermoanerobacterium thermosaccharolyticum comb. nov.