Determination of the primary structures of 16 asialo-carbohydrate units derived from human plasma α1-acid glycoprotein by 360-MHz 1H NMR spectroscopy and permethylation analysis
Determination of the primary structures of 16 asialo-carbohydrate units derived from human plasma α1-acid glycoprotein by 360-MHz 1H NMR spectroscopy and permethylation analysis
复制标题
通过 360 MHz 1H NMR 光谱和全甲基化分析测定源自人血浆 α1-酸性糖蛋白的 16 个脱唾液酸碳水化合物单元的一级结构
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
K. Schmid
中科院分区：
文献类型：
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作者：
B. Fournet;J. Montreuil;G. Strecker;L. Dorland;J. Haverkamp;J. Vliegenthart;J. Binette;K. Schmid
发起本研究是为了确定
碳水化合物单元的一级结构
去唾液酸人血浆α-酸性糖蛋白。在较早的
研究源自四种的 I6 糖肽
该蛋白质的五个糖基化位点在
均质状态。自从阐明了结构
如此大量的碳水化合物单元
化学和酶促程序需要很长的时间
经过一段时间和大量的材料，新的
方法-360-MHz H NMR 光谱-组合
引入甲基化分析和部分丙酮解
在本研究中。这种高度灵敏的方法允许
阐明碳水化合物单元的结构
提到糖肽要在很短的时间内进行。目前的调查显示，该结构
16 种杂聚糖可分为五类，并且，
除两类外，其他结构都是新的。两个
指定为 A 和 B 的类别具有已知的二元和三元
结构，分别。第三类指定为C
已证明其结构是四触角的，而其他
与指定的 BF 和 CF 类相比
B 类和 C 类结构，其特征是附加
岩藻糖残基处于迄今未知的位置和连接。
后一个单糖残基连接在 a(1-3) 中
与 Gal@(1 +4) 相邻的键，与 GlcNAc 残基 7 相连。
本研究还表明，每个糖基化位点
汇集的α]-酸性糖蛋白具有碳水化合物单元
不同的结构。
The present study was initiated in order to determine
the primary structures of the carbohydrate units of
desialyzed human plasma al-acid glycoprotein. In an earlier
investigation I6 glycopeptides which originated from four of
the five glycosylation sites of this protein were prepared in the
homogeneous state. Since the elucidation of the structures of
such a large number of carbohydrate units by the conventional
chemical and enzymatic procedures would require a very long
period of time and considerable amounts of material, a new
method-360-MHz H NMR spectroscopy-in combination
with methylation analysis and partial acetolysis was introduced
i n the present study. This highly sensitive method allowed the
elucidation of the structures of the carbohydrate units of the
mentioned glycopeptides to be carried out in a very short period of time. The present investigation revealed that the structures
of the 16 heteroglycans can be grouped into five classes, and,
except for those of two classes, the structures are new. Two
classes designated A and B possess the known bi- and trimtennary
structures, respectively. A third class designated C
proved to be tetraantennary in its structure, whereas the other
classes designated BF and CF are, in comparison with the
structures of classes B and C, characterized by an additional
fucose residue in a hitherto unknown position and linkage.
The latter monosaccharide residue is attached in an a( 1-3)
bond, vicinal to Gal@(1 +4), to the GlcNAc residue 7. The
present study also revealed that each glycosylation site of
pooled a]-acid glycoprotein possesses carbohydrate units with
different structures.