Effect of crude lipopolysaccharide from Escherichia coli O127:B8 on the amebocyte-producing organ of Biomphalaria glabrata (Mollusca)
Effect of crude lipopolysaccharide from Escherichia coli O127:B8 on the amebocyte-producing organ of Biomphalaria glabrata (Mollusca)
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DOI：
10.1016/j.dci.2011.03.032
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发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Salamat, Zahra
中科院分区：
文献类型：
--
作者：
Sullivan, John T.;Bulman, Christina A.;Salamat, Zahra
脂多糖（LPS）是一种病原体相关分子模式（PAMP），脊椎动物和无脊椎动物的内部防御系统（IDS）都对之产生反应。我们测量的有丝分裂反应的造血组织的的染色体传播蜗牛，光滑双脐螺，粗脂多糖从大肠杆菌O 127：B8。在剂量反应研究中，用一系列浓度的粗LPS注射蜗牛，并用0.1%秋水仙素处理6 h后，在注射后24 h固定的阿米巴细胞产生器官（APO）的组织切片中计数有丝分裂像。在从萨尔瓦多株蜗牛，这是遗传抵抗感染曼氏血吸虫，LPS浓度为0.01毫克/毫升及以上引发了大量的有丝分裂活性的增加，而在APOs从过敏体质的NIH白化病蜗牛，浓度为0.1毫克/毫升引起了一个小得多，但统计学上显着的增加。一项时间进程研究，没有秋水仙碱治疗，显示在萨尔瓦多APOs的有丝分裂反应0.1毫克/毫升发生的18小时PI，在24小时达到峰值，并返回到控制水平的72小时;美国国立卫生研究院白化病APOs显示没有检测到的反应。当萨尔瓦多载脂蛋白在体外暴露于粗LPS，没有增加有丝分裂活动发生，结果表明可能需要外周组织或血淋巴因子。由粗LPS诱导的增加的细胞增殖代表无脊椎动物IDS对来自革兰氏阴性细菌的一种或多种PAMP的新的全身性应答。(C)2011爱思唯尔有限公司版权所有。
Lipopolysaccharide (LPS) is a pathogen associated molecular pattern (PAMP) to which the internal defense system (IDS) of both vertebrates and invertebrates responds. We measured the mitotic response of the hematopoietic tissue of the schistosome-transmitting snail, Biomphalaria glabrata, to crude LPS from Escherichia coli O127:B8. In a dose-response study, snails were injected with a range of concentrations of crude LPS, and mitotic figures were enumerated in histological sections of amebocyte-producing organ (APO) fixed at 24h post-injection (PI) following a 6h treatment with 0.1% colchicine. In APOs from Salvador strain snails, which are genetically resistant to infection with Schistosoma mansoni, LPS concentrations of 0.01 mg/ml and above triggered a large increase in mitotic activity, whereas in APOs from schistosome-susceptible NIH albino snails, concentrations of 0.1 mg/ml elicited a much smaller, but statistically significant increase. A time course study, without colchicine treatment, revealed that in Salvador APOs the mitotic response to 0.1 mg/ml occurred by 18 h PI, peaked at 24 h, and returned to control levels by 72 h; NIH albino APOs showed no detectible response. When Salvador APOs were exposed to crude LPS in vitro, no increase in mitotic activity occurred, a result suggesting the possible requirement for a peripheral tissue or hemolymph factor. The increased cell proliferation induced by crude LPS represents a novel systemic response of an invertebrate IDS to one or more PAMPs from a Gram-negative bacterium. (C) 2011 Elsevier Ltd. All rights reserved.