Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates
Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates
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DOI：
10.1128/jcm.42.10.4672-4678.2004
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发表时间：
2004-10-01
影响因子：
9.4
通讯作者：
Liebana, E
中科院分区：
文献类型：
--
作者：
Martinez-Urtaza, J;Lozano-Leon, A;Liebana, E
尽管海产品受到副溶血性弧菌污染存在潜在风险，但缺乏关于欧洲致病性副溶血性弧菌发生情况的信息。这种细菌是1999年在西班牙加利西亚发生的与生牡蛎食用有关的大规模疫情(病例住进一家医院)中分离出来的，加利西亚是世界上最重要的贝类生产地区之一。检测了1999年加利西亚疫情中的两株副溶血性弧菌，同期从西班牙医院获得的另外三株临床分离株，临床来源的两株参考菌株，以及西班牙环境中的五株分离株。亚洲和北美分离的17株属于大流行克隆的菌株被包括在研究中进行比较。对所有分离株进行了血清学分型、毒力相关基因的聚合酶链式反应(PCR)、脉冲场凝胶电泳法(PFGE)和质粒分析。来自西班牙医院的5株临床分离株中有4株属于O4：K11型；其余分离株为O4：K不可分型(KUT)。所有五个分离株都对副溶血性弧菌毒素R和tlh(种特异性基因)和tdh呈阳性，而trh和组特异性聚合酶链式反应(一种检测大流行克隆的聚合酶链式反应方法)均为阴性。用NOTI进行PFGE分析，仍可将欧洲分离物区分为两个紧密相关的PFGE类型，包括在一个同源聚类群中，明显区别于亚洲和北美分离物。5株环境分离株分别属于O2：K28、O2：KUT、O3：K53、O4：KUT和O8：K22血清型，毒力基因均为阴性。这五个分离物被区分为五个不同的PFGE类型，与研究中包括的任何其他分离物都不相关。虽然西班牙临床分离株的毒力特征(tdh阳性，trh阴性)与来自亚洲和北美的O3：K6克隆的毒力特征一致，但通过组特异性PCR、PFGE和血清分型，它们显然被排除在该克隆之外。这项研究的结果表明，一个独特而特异的克隆可能与欧洲副溶血性弧菌的感染有关。
In spite of the potential risk involved with contamination of seafood with Vibrio parahaemolyticus, there is a lack of information on the occurrence of pathogenic V. parahaemolyticus in Europe. This organism was isolated in 1999 from a large outbreak (64 cases admitted to a single hospital) associated with raw oyster consumption in Galicia, Spain, one of the most important regions in shellfish production worldwide. Two V. parahaemolyticus isolates from the 1999 Galicia outbreak, three additional clinical isolates obtained in the same period from hospitals in Spain, two reference strains from clinical sources, and five Spanish environmental isolates were examined. Seventeen isolates belonging to the pandemic clone isolated in Asia and North America were included in the study for comparison. All isolates were characterized by serotyping, PCR for virulence-related genes, pulsed-field gel electrophoresis (PFGE), and plasmid analysis. Four of the five clinical isolates from hospitals in Spain belonged to serotype O4:K11; the remaining isolate was O4:K untypeable (KUT). All five isolates were positive for V. parahaemolyticus toxR and tlh (species-specific genes) and tdh and negative for trh and group-specific PCR (a PCR method for detection of the pandemic clone). PFGE analysis with NotI and Still discriminated the European isolates in two closely related PFGE types included in a homogeneous cluster, clearly differentiated from the Asian and North American isolates. The five environmental isolates belonged to serotypes O2:K28, O2:KUT, O3:K53, O4:KUT, and O8:K22 and were negative for all virulence genes. The five isolates were discriminated into five different PFGE types unrelated to any other isolate included in the study. While the virulence characteristics (tdh positive, trh negative) of the Spanish clinical isolates matched those of the O3:K6 clone from Asia and North America, they were clearly excluded from this clone by group-specific PCR, PFGE, and serotyping. The results of this study suggest that a unique and specific clone could be related to the V. parahaemolyticus infections in Europe.