New roles for cis-jasmone as an insect semiochemical and in plant defense
New roles for cis-jasmone as an insect semiochemical and in plant defense
复制标题
DOI：
10.1073/pnas.160241697
复制
发表时间：
2000-08-01
影响因子：
11.1
通讯作者：
Woodcock, CM
中科院分区：
文献类型：
--
作者：
Birkett, MA;Campbell, CAM;Woodcock, CM
顺式茉莉酮，或称(Z)-茉莉酮，是众所周知的植物挥发物的一种成分，它的释放可以由损害引起，例如在昆虫食草期间。利用莴苣蚜虫的嗅觉系统来研究这种昆虫所避免的植物的挥发物，在实验室选择测试中发现(Z)-茉莉素具有电生理活性，并且具有驱避作用。在田间研究中，诱捕器对稻花蚜有驱避作用，在冬小麦田块中，(Z)-茉莉素处理可减少稻花蚜的数量。在室内和风洞试验中，对七斑瓢虫和一种蚜虫具有拮抗作用的昆虫发现了引诱剂的活性。当将(Z)-茉莉素以蒸汽相的方式施用于完整的豆类植物时，(Z)-茉莉素诱导挥发性化合物的产生，包括单萜烯(E)- β -辛烯，它会影响植物的防御，例如通过刺激寄生昆虫的活动。在(Z)-茉莉停止作用48 h后，这些植物对风洞中蚜虫的吸引力更大。这种可能的信号作用(Z)-茉莉素与生物合成相关的茉莉酸甲酯在质上不同，并且在去除刺激后具有持久的作用。采用差异显示法比较了(Z)-茉莉素和茉莉酸甲酯蒸气对大豆叶片mRNA群体的影响。克隆了一个差异显示片段，并通过Northern blotting显示(Z)-茉莉素在叶片组织中表达上调。该序列经同源性鉴定为源自编码a-微管蛋白异构体的基因。
cis-Jasmone, or (Z)-jasmone, is well known as a component of plant volatiles, and its release can be induced by damage, for example during insect herbivory. Using the olfactory system of the lettuce aphid to investigate volatiles from plants avoided by this insect, (Z)-jasmone was found to be electrophysiologically active and also to be repellent in laboratory choice tests. In field studies, repellency from traps was demonstrated for the damson-hop aphid, and with cereal aphids numbers were reduced in plots of winter wheat treated with (Z)-jasmone. In contrast, attractant activity was found in laboratory and wind tunnel tests for insects acting antagonistically to aphids, namely the seven-spot ladybird and an aphid parasitoid. When applied in the vapor phase to intact bean plants, (Z)-jasmone induced the production of volatile compounds, including the monoterpene (E)-beta-ocimene, which affect plant defense, for example by stimulating the activity of parasitic insects. These plants were more attractive to the aphid parasitoid in the wind tunnel when tested 48 h after exposure to (Z)-jasmone had ceased. This possible signaling role of (Z)-jasmone is qualitatively different from that of the biosynthetically related methyl jasmonate and gives a long-lasting effect after removal of the stimulus. Differential display was used to compare mRNA populations in bean leaves exposed to the vapor of (Z)-jasmone and methyl jasmonate. One differentially displayed fragment was cloned and shown by Northern blotting to be up-regulated in leaf tissue by (Z)-jasmone. This sequence was identified by homology as being derived from a gene encoding an a-tubulin isoform.