Utilization of Oral Sucrose Load During Exercise in Humans: Effect of the α-Glucosidase Inhibitor Acarbose
Utilization of Oral Sucrose Load During Exercise in Humans: Effect of the α-Glucosidase Inhibitor Acarbose
复制标题
人类运动期间口服蔗糖负荷的利用：α-葡萄糖苷酶抑制剂阿卡波糖的作用
DOI：
10.2337/diab.35.11.1294
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发表时间：
1986
期刊：
影响因子：
7.7
通讯作者：
P. Lefèbvre
中科院分区：
文献类型：
--
作者：
J. Gerard;B. Jandrain;F. Pirnay;N. Pallikarakis;G. Krzentowski;M. Lacroix;F. Mosora;A. Luyckx;P. Lefèbvre
我们研究了健康志愿者在适应中等强度（50% Vmaxo2）长时间（4小时）运动15分钟后，对100克蔗糖负荷的激素和代谢反应。研究了α-葡萄糖苷酶抑制剂阿卡波糖100 mg剂量对蔗糖负荷的影响。使用“自然标记的[13C]蔗糖”来跟踪同位素比质谱法将摄入的糖转化为过期空气中的二氧化碳。收集了9名受试者的循环激素和代谢物数据，并对其中6名受试者采用了间接量热法和稳定同位素方法。在安慰剂组，运动4小时内，93±4 g蔗糖被完全氧化，总碳水化合物利用率为235±14 g，内源性碳水化合物利用率为142±13 g，总脂质氧化为121±7 g。单次口服100 mg阿卡波糖并没有显著改变总碳水化合物（239±2 g/4 h）或脂质（122±6 g/4 h）氧化。相比之下，蔗糖氧化降低到53±6 g/4 h，内源性碳水化合物利用率增加到186±7 g/4 h。阿卡波糖降低血糖和果糖的升高以及血浆胰岛素和C肽的升高证实了这些作用，而较低的丙氨酸循环水平表明糖异生率较高。这些数据表明，运动开始后不久摄入的100克葡萄糖负荷是一种完美的可利用代谢底物。此外，同时摄入100毫克阿卡波糖会显著减少运动中蔗糖的可用性，如果这种或其他具有类似性质的化合物被设想用于治疗糖尿病患者，则必须考虑这一发现。
We investigated the hormonal and metabolic response to a 100-g sucrose load given 15 min after adaptation to moderate-intensity (50% Vmaxo2) long-duration (4-h) exercise in healthy volunteers. The effect of a 100-mg dose of the α-glucosidase inhibitor Acarbose ingested with the sucrose load was also investigated. “Naturally labeled [13C] sucrose” was used to follow the conversion to expired-air CO2 of the sugar ingested by isotope-ratio mass spectrometry. Circulating hormone and metabolite data were obtained in nine subjects, and indirect calorimetry and stable isotope methodology were applied to six of them. Under placebo, 93 ± 4 g sucrose were entirely oxidized during the 4 h of exercise, total carbohydrate utilization was 235 ± 14 g, endogenous carbohydrate utilization was 142 ± 13 g, and total lipid oxidation was 121 ± 7 g. A single oral dose of 100 mg Acarbose ingested with the sucrose load did not significantly modify total carbohydrate (239 ± 2 g/4 h) or lipid (122 ± 6 g/4 h) oxidation. In contrast, sucrose oxidation was reduced to 53 ± 6 g/4 h and endogenous carbohydrate utilization increased to 186 ± 7 g/4 h. Reduction of the rises in blood glucose and fructose and of the increases in plasma insulin and C peptide under Acarbose confirmed these effects, whereas lower circulating levels of alanine suggested a higher rate of gluconeogenesis. These data show that a 100-g glucose load ingested soon after initiation of exercise is a perfect available metabolic substrate. Furthermore, the simultaneous ingestion of 100 mg Acarbose significantly reduces the availability of sucrose during exercise, a finding that has to be considered if this or other compounds with similar properties are envisaged for the treatment of diabetic patients.