Correlation between specific IgM levels and percentage IgG-class antibody avidity to Toxoplasma gondii
Correlation between specific IgM levels and percentage IgG-class antibody avidity to Toxoplasma gondii
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DOI：
10.1590/s0036-46652008000400010
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发表时间：
2008-08-01
期刊：
影响因子：
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通讯作者：
Granato, Celso F. H.
中科院分区：
文献类型：
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作者：
Leite, Marcel;Siciliano, Sonia;Granato, Celso F. H.
弓形虫病通常是无症状的世界性播散性感染。在其先天性表现，它可能会导致流产或胎儿畸形。产前评估被认为是至关重要的，以确定血清阴性的妇女，并允许预防。IgM检测灵敏度的最新改进使得IgM检测成为所有极度延长的急性期标志物。IgG亲合力评价试验成为必要。观察表明，在I-M水平和亲合力百分比之间建立了相关性呼叫。这表明亲合力试验通常可能不是必需的。本文分析了202例弓形虫IgM抗体水平及其IgG抗体亲合力百分率，以确定特异性IgM抗体水平，其IgM抗体定量本身就能确定血清学诊断，从而避免了抗体亲合力的测定。我们发现，IgM水平低于2.0和高于6.0的弓形虫病的血清学诊断，可以建立不需要IgG亲和力试验。这两个参数之间的IgM水平与不同的亲合力指数有关，突出了其评价作为确认弓形虫病的一种手段的重要性。在此演示之后，可以避免75%的情况下的亲合力测试，以减少周转时间并降低成本。
Toxoplasmosis is all usually asymptomatic worldwide disseminated infection. In its congenital presentation it may lead to abortion or fetal malformations. Antenatal evaluation is considered of paramount importance to identify seronegative women and allow for prophylaxis. Recent improvements in sensitivity of IgM tests has made IgM detection all extremely protracted acute phase marker. and IgG avidity evaluation test became necessary. Observation has shown that a correlation call be established between I-M levels and avidity percentages. suggesting that frequently the avidity test may not be necessary. In this study we analyzed Toxoplasma gondii IgM levels of 202 samples and their IgG avidity percentages, in order to define specific levels whose IgM quantification Could by itself define serodiagnosis and therefore make the avidity evaluation unnecessary. We showed that for IgM levels bellow 2.0 and above 6.0 serodiagnosis of toxoplasmosis could be established without need of IgG avidity test. IgM levels between these two parameters are associated with varying avidity indexes highlighting the importance of its evaluation as a means to confirm toxoplasmosis. Following this demonstration it was possible to avoid the avidity test for 75% of the cases, to reduce the turnaround time and to reduce costs.