Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene
Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene
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DOI：
10.1093/pcp/pcm164
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发表时间：
2008-01-01
影响因子：
4.9
通讯作者：
Esaka, Muneharu
中科院分区：
文献类型：
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作者：
Badejo, Adebanjo A.;Tanaka, Nobukazu;Esaka, Muneharu
GDP-D-甘露糖焦磷酸化酶（GMP）是植物抗坏血酸（阿萨）合成的Smirnoff-Wheelers途径中的一个重要酶。我们最近报道了金虎尾GMP基因的表达，命名为MgGMP，与植物的阿萨含量相关。针叶树植物相对于更好研究的模式植物如拟南芥具有非常高水平的阿萨。在这里，我们发现，GMPmRNA水平在针叶树高于拟南芥和番茄。此外，uidA报告基因在烟草培养物的原生质体中的瞬时表达显示MgGMP基因启动子具有比花椰菜花叶病毒35 S和拟南芥GMP启动子更高的活性。表达MgGMP基因及其启动子的转基因烟草植株的阿萨含量比野生型高约2倍。
GDP-D-mannose pyrophosphorylase (GMP) is an important enzyme in the Smirnoff-Wheelers pathway for the biosynthesis of ascorbic acid (AsA) in plants. We have reported recently that the expression of the acerola (Malpighia glabra) GMP gene, designated MgGMP, correlates with the AsA content of the plant. The acerola plant has very high levels of AsA relative to better studied model plants such as Arabidopsis. Here we found that the GMP mRNA levels in acerola are higher than those from Arabidopsis and tomato. Also, the transient expression of the uidA reporter gene in the protoplasts of Nicotiana tabacum cultures showed the MgGMP gene promoter to have higher activity than the cauliflower mosaic virus 35S and Arabidopsis GMP promoters. The AsA content of transgenic tobacco plants expressing the MgGMP gene including its promoter was about 2-fold higher than that of the wild type.