Simultaneous determination of hydroxymethylbilane synthase and uroporphyrinogen III synthase in erythrocytes by high-performance liquid chromatography.
Simultaneous determination of hydroxymethylbilane synthase and uroporphyrinogen III synthase in erythrocytes by high-performance liquid chromatography.
复制标题
高效液相色谱法同时测定红细胞中羟甲基胆烷合酶和尿卟啉原Ⅲ合酶。
作者：
D. Wright;C. Lim
建立了一种同时测定红细胞羟甲基丁二烷合酶和尿卟啉原III合酶活性的高效液相色谱方法。有效地分离尿卟啉I和III异构体，可以准确地定量单独的异构体和总的尿卟啉浓度。尿卟啉总产量用于计算羟甲基双环丙烷合酶活性，形成的尿卟啉III的量代表尿卟啉原III合酶的活性。确定了两种酶的正常范围。
A high-performance-liquid-chromatographic method is developed for the simultaneous determination of hydroxymethylbilane synthase and uroporphyrinogen III synthase activity in erythrocytes. Effective separation of uroporphyrin I and III isomers allows the accurate quantification of individual isomers and the total uroporphyrin concentration. Total uroporphyrin production is used to calculate hydroxymethylbilane synthase activity, and the amount of uroporphyrin III formed represents the activity of uroporphyrinogen III synthase. Normal ranges are established for the two enzymes.