KINETICS OF SYNTHESIS OF NITROGENASE IN BATCH AND CONTINUOUS CULTURE OF ANABAENA-FLOS-AQUAE
KINETICS OF SYNTHESIS OF NITROGENASE IN BATCH AND CONTINUOUS CULTURE OF ANABAENA-FLOS-AQUAE
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DOI：
10.1007/bf00410125
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
BONE, DH
中科院分区：
文献类型：
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作者：
BONE, DH
在间歇培养和铁、磷、尿素限制的连续培养中，研究了水华鱼腥藻固氮酶的氧灭活和活力恢复。在分批培养中，刀豆氨酸，氯霉素，甲胺，原黄素，嘌呤霉素和尿素抑制恢复过程。固氮酶活性的恢复率与光照强度、尿素、铵盐和硝酸盐浓度有关，与生长速率无关。在连续培养中，氧气和尿素导致固氮酶失活。
Nitrogenase ofAnabaena flos-aquaewas inactivated by oxygen and recovery of activity was measured in batch and iron, phosphate and urea-limited continuous cultures. In batch culture, canavanine, chloramphenicol, methylamine, proflavine, puromycin and urea inhibited the recovery process. The rate of recovery of nitrogenase activity in continuous cultures was dependent on light intensity, concentration of urea, ammonium salts and nitrate, and independent of growth rate. Oxygen and urea caused an inactivation of nitrogenase in continuous cultures.