Arrangement of connective tissue components in the walls of seminiferous tubules of man and monkey.
Arrangement of connective tissue components in the walls of seminiferous tubules of man and monkey.
复制标题
人和猴子生精小管壁中结缔组织成分的排列。
作者：
L. Hermo;M. Lalli;Y. Clermont
在灵长类动物中，将生精上皮与间质分开的膜由一到三层（猴）或两到六层（人）肌样细胞与一到两层纤维细胞样外膜细胞相关。所有这些细胞都通过充满各种结缔组织细胞间成分的不规则空间彼此分开。生精上皮成分下方是连续的、通常是多余的基底膜。类似的基底膜样材料在肌样细胞质膜的小区域附近和上方形成一层。在所有结缔组织层中都可以看到聚集成各种大小的束的胶原原纤维，但在生精上皮和肌样细胞最内层之间的空间中特别丰富。 Verhoeff 铁苏木精技术证明的弹性原纤维也存在。弹性原纤维由均质材料组成，是短的、不规则的、分支实体，其直径与胶原原纤维相当或更小。此外，各种结缔组织层中存在大量直径为 12-15 nm 的微纤维。这些微纤维具有浓密染色的皮质和浅色染色的核心。当靠近肌样细胞观察时，微纤维束似乎插入质膜旁边明确的区域。这些区域通常面向在肌样细胞质膜内部观察到的电子致密材料斑块，肌动蛋白丝插入其中。微纤维束通常跨越同一层以及相邻层的肌样细胞之间的间隙。微原纤维也与弹性原纤维的表面密切相关，并且与胶原原纤维交织在一起。因此，微原纤维似乎将相邻的肌样细胞桥接并结合在一起，并将这些细胞的表面锚定到存在于限制膜的细胞间隙中的弹性纤维束和胶原原纤维上。
In primates the membrane separating the seminiferous epithelium from the interstitial space is composed of one to three (monkey) or two to six layers (man) of myoid cells associated with one to two layers of fibrocyte-like adventitial cells. All these cells are separated from each other by irregular spaces filled with various connective tissue intercellular components. Subjacent to the elements of the seminiferous epithelium is a continuous, often redundant, basement membrane. A similar basement membrane-like material forms a layer next to and over small areas of the plasma membrane of myoid cells. Collagen fibrils grouped in bundles of various sizes are seen in all connective tissue layers but are particularly abundant in the space between the seminiferous epithelium and the innermost layer of myoid cells. Elastic fibrils demonstrated by the Verhoeff iron hematoxylin technique are also present. Composed of a homogeneous material, the elastic fibrils are short, irregular, branching entities with a diameter comparable to or smaller than that of collagen fibrils. In addition, an abundance of microfibrils with a diameter of 12-15 nm is present in the various connective tissue layers. These microfibrils have a densely stained cortex and a lightly stained core. When seen close to the myoid cells, bundles of micro fibrils appear to insert on well defined areas next to the plasma membrane. These areas commonly face the patches of electron-dense material observed on the inner aspect of the plasma membrane of the myoid cells and in which the actin filaments are inserted. Bundles of microfibrils often span the gap between myoid cells of the same layer as well as those of adjacent layers. Microfibrils are also closely related to the surface of elastic fibrils and are seen intertwining with collagen fibrils. Thus microfibrils appear to bridge and bind together adjacent myoid cells and anchor the surface of these cells to the bundles of elastic and collagen fibrils present in the intercellular spaces of the limiting membrane.