Monocyte chemoattractant protein 1 (MCP-1) gene polymorphism is not associated with severe and cerebral malaria in Thailand.
Monocyte chemoattractant protein 1 (MCP-1) gene polymorphism is not associated with severe and cerebral malaria in Thailand.
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DOI：
10.7883/yoken.jjid.2006.239
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发表时间：
2006-08
影响因子：
2.2
通讯作者：
N. Dechkum;H. Hananantachai;J. Patarapotikul;J. Ohashi;S. Krudsood;S. Looareesuwan;K. Tokunaga
中科院分区：
文献类型：
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作者：
N. Dechkum;H. Hananantachai;J. Patarapotikul;J. Ohashi;S. Krudsood;S. Looareesuwan;K. Tokunaga
恶性疟原虫感染引起的脑型疟疾的发病机制被认为与中枢神经系统的炎症有关。由于单核细胞趋化蛋白1 （MCP-1）是一种强烈参与炎症过程的趋化因子，我们在这里研究了MCP-1基因多态性与泰国严重或脑型疟疾的关系。将泰国西北部的疟疾患者分为轻度（206例）、重度（165例）和脑型（110例）疟疾病例组。通过PCR-RFLP、PCR-SSP或直接测序分析了启动子（-2518A/G、-2348G/C、-2158C/T、-2076A/T和-2072T/C）上的5个单核苷酸多态性（SNP）和内含子1 （764C/G）上的1个SNP。SNP -2158是本研究新发现的多态性。所有snp的基因型和等位基因频率在轻度和重度疟疾、轻度和脑型疟疾之间无显著差异。4个snp （-2518A/G、-2348G/C、-2076A/T和764C/G）存在强烈的连锁不平衡，形成4种主要的单倍型。最常见的单倍型是GGAC。结果表明，MCP-1基因多态性与疟疾严重程度无关，这意味着MCP-1不是导致该泰国人群疟疾严重程度的原因。
The pathogenesis of cerebral malaria from Plasmodium falciparum infection is thought to involve inflammation of the central nervous system. Since monocyte chemoattractant protein 1 (MCP-1) is a chemokine strongly involved in the inflammatory process, we here study MCP-1 gene polymorphisms in association with severe or cerebral malaria in Thailand. Malaria patients in the northwest of Thailand were grouped into mild (n=206), severe (165), and cerebral (110) malaria case groups. Five single nucleotide polymorphisms (SNPs) in the promoter (-2518A/G, -2348G/C, -2158C/T, -2076A/T, and -2072T/C), and 1 SNP in intron 1 (764C/G) were analyzed by PCR-RFLP, PCR-SSP, or direct sequencing. The SNP -2158 was a novel polymorphism found in this study. For all SNPs, genotype and allele frequencies were not significantly different between mild and severe or mild and cerebral malaria. Strong linkage disequilibrium was found among 4 SNPs (-2518A/G, -2348G/C, -2076A/T, and 764C/G), resulting in 4 major estimated haplotypes. The most common haplotype was GGAC. The results indicated that MCP-1 gene polymorphisms were not associated with malaria severity, implying that MCP-1 was not a cause of malaria severity in this Thai population.