Control of messenger RNA concentration by differential cytoplasmic half-life. Adenovirus messenger RNAs from transcription units 1A and 1B.
Control of messenger RNA concentration by differential cytoplasmic half-life. Adenovirus messenger RNAs from transcription units 1A and 1B.
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通过不同的细胞质半衰期控制信使 RNA 浓度。
DOI：
10.1016/0022-2836(81)90537-4
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发表时间：
1981
影响因子：
5.6
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
--
作者：
Wilson,MC;DarnellJr,JE
在腺病毒2裂解周期期间，已经检查了几种特定病毒信使RNA丰度变化的基础。位于病毒基因组左端的两个独立转录单位1A和1B产生5′和3′共末端mRNA组，其在感染后不同时间的相对胞质浓度不同。然而，从这些转录单位的核多聚腺苷酸化，剪接分子的形成，似乎并没有改变与细胞质mRNA的变化相称。所有形式的1A和1B mRNA都可以被发现为核聚腺苷酸化分子，在任何时候都具有相似的相对量。通过测量标记的mRNA累积到最大水平的速率，确定在感染早期，这些mRNA具有短的细胞质半衰期，大约为6至10分钟。在DNA复制后，来自1A区的两个早期mRNA和来自1B区的较短mRNA 1B1040的稳定性增加了约10倍。来自区域1B，1B2300的较长mRNA不具有增加的细胞质半衰期。由于半衰期的变化，两种1B mRNA的相对稳态丰度在感染后期被逆转。增加的稳定性也被认为是1A500 mRNA在感染后期出现的原因。这种mRNA在早期检测不到，除非蛋白质合成被放线菌酮抑制。这些结果指出了mRNA稳定性控制的重要性，需要持续的RNA和蛋白质合成，在真核生物中的一些基因的表达调控。
The basis for the change in abundance of several specific viral messenger RNAs has been examined during the adenovirus 2 lytic cycle. The two independent transcription units, 1A and 1B, at the left end of the viral genome produce groups of 5′ and 3′ co-terminal mRNAs which vary in relative cytoplasmic concentration at different times after infection. The formation of nuclear polyadenylated, spliced molecules from these transcription units, however, does not appear to vary commensurate with the cytoplasmic mRNA changes. All forms of the 1A and 1B mRNAs could be found as nuclear polyadenylated molecules in similar relative amounts at all times. By measuring the rate of accumulation of labeled mRNA to maximal levels, it was determined that early during infection these mRNAs have short cytoplasmic half-lives, on the order of six to ten minutes. After DNA replication there is an approximately tenfold increase in the stability of the two early mRNAs from region 1A and the shorter mRNA from the 1B region, 1B1040. The longer mRNA from region 1B, 1B2300, does not have an increased cytoplasmic half-life. Because of the changing half-lives the relative steady-state abundance of the two 1B mRNAs is reversed late in infection. Increased stability is also suggested as the cause of the appearance of the 1A500mRNA late in infection. This mRNA is not detected at early times unless protein synthesis is inhibited with cycloheximide. These results point to the importance of the control of mRNA stability, requiring both continued RNA and protein synthesis, for the regulated expression of some genes in eukaryotes.