Nuclear magnetic resonance studies of the interaction of alamethicin with lecithin bilayers.
Nuclear magnetic resonance studies of the interaction of alamethicin with lecithin bilayers.
复制标题
阿拉甲辛与卵磷脂双层相互作用的核磁共振研究。
DOI：
10.1021/bi00721a010
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发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
S. Chan
中科院分区：
文献类型：
--
作者：
A. Lau;S. Chan
用核磁共振和电子显微镜研究了丙甲霉素与非超声处理的卵磷脂多层膜和超声处理的双层囊泡的相互作用。已表明丙甲霉素是一种表面活性剂，其主要与丙甲霉素相互作用（Payne等人，1970），一种来自真菌绿色木霉（Trichoderma viride）的胞外大环多肽（Meyer和Reusser，1967），已显示诱导离子穿过某些生物膜移动（Pressman，1968）。还表明，这种抗生素与黑色脂质膜相互作用，形成具有离散电导状态的电压门控离子通道（Muller和Rudin，1968; Gordon和Flaydon，1972）。由于不知道丙甲霉素本身会易位穿过黑色脂质膜，因此已经提出丙甲霉素诱导电压可门控离子孔（Eisenberg et al.，1973年）。了解丙甲霉素与磷脂双层膜的相互作用模式对了解这种抗生素的作用是必不可少的。可以使用各种方法来阐明这种相互作用，包括电测量（Eisenberg等人，1973; Lau和Hall，1974）、X射线衍射和差示扫描量热法（Chapman等人，1969）、电子自旋共振（esr）自旋标记（Finer等人，1969; Levine等人，1973）、圆二色性（McMullen等人，1971）和荧光技术（Case等人，1974年）。然而，核磁共振（nmr）光谱由于其对结构细节和环境变化的敏感性而提供了独特的优势。该灵敏度由帕萨迪纳市加州理工学院亚瑟阿莫斯诺伊斯化学物理实验室提供，编号4836。加州91125. 1974年4月8日收到。这项工作得到了国家普通医学科学研究所的LS公共卫生服务赠款GM-14523的支持。
The interaction of alamethicin with both un-sonicated lecithin multilayers and sonicated bilayer vesicles has been investigated by nuclear magnetic resonance(nmr) spectroscopy and electron microscopy. It is shown that alamethicin is a surface active agent, which interacts primarily i^ Llamethicin (Payne et al., 1970), an extracellular ma-crocyclic polypeptide from the fungus Trichoderma viride (Meyer and Reusser, 1967), has been shown to induce ion movements across certain biological membranes (Pressman, 1968). It has also been shown that this antibiotic interacts with black lipid membranes to form voltage gateable ion channels with discrete conductance states (Muller and Rudin, 1968; Gordon and Flaydon, 1972). Since alamethi-cin itself is not known to be translocated across a black lipid membrane, it has been proposed that voltage gateable ion pores are induced by alamethicin (Eisenberg et al., 1973). A knowledge of the mode of interaction of alamethicin with phospholipid bilayer membranes is essential toward the understanding of the action of this antibiotic. Various methods may be used to elucidate this interaction, including electrical measurements (Eisenberg et al., 1973; Lau and Hall, 1974), X-ray diffraction and differential scanning cal-orimetry (Chapman et al., 1969), electron spin resonance (esr) spin labeling (Finer et al., 1969; Levine et al., 1973), circular dichroism (McMullen et al., 1971), and fluores-cence techniques (Case et al., 1974). Nuclear magnetic res-onance (nmr) spectroscopy, however, offers unique advantages by virtue of its sensitivity towardunravelling structural details and environmental changes. This sensitivity, for f Contribution No. 4836 from the Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena. California 91125. Received April 8, 1974. This work was supported by LS Public Health Service Grant GM-14523 from the National Insti-tute of General Medical Sciences.