Enhancing activity of N-glycosylation for constitutive proteins secretions in non-polarized cells
Enhancing activity of N-glycosylation for constitutive proteins secretions in non-polarized cells
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DOI：
10.1016/j.bbrc.2009.02.101
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发表时间：
2009-04-17
影响因子：
3.1
通讯作者：
Saito, Saburo
中科院分区：
文献类型：
--
作者：
Akiyama, Nobutake;Ohno, Yuji;Saito, Saburo
小鼠白细胞介素2(MILs)与增强型绿色荧光蛋白(EGFP)的融合蛋白在成纤维细胞和上皮细胞中均有表达。在这些蛋白质中，mIL-31衍生物以N-糖基化依赖的方式最有效地分泌到培养基中。从缺失突变体的分析，构成分泌物的最小结构由一个信号肽和N-糖基化组成。将mIL-31的信号序列导入到人P53蛋白中，不能分泌产物，但进一步增加N-糖基化位点导致具有生物活性的P53蛋白以N-糖基化形式结构性分泌到培养液中。在这份报告中，我们展示了N-糖基化对构成蛋白分泌的重要性，特别是使用非极化细胞。(C)2009 Elsevier Inc.保留所有权利
Several fusion proteins of mouse Interleukins (mILs) and the enhanced green fluorescent protein (EGFP) were expressed in fibroblast and epithelial cells. Among these proteins, the mIL-31 derivative was the most efficiently secreted into the medium in a N-glycosylation-dependent manner. From the analysis of deletion mutants, the minimal structure for constitutive secretions consisted of a signal peptide and N-glycosylation. Introduction of the signal sequence from mIL-31 to human p53 protein failed to secrete the products, but further addition of the N-glycosylation site resulted in constitutive secretion of biologically active p53 protein into the medium in the N-glycosylated form. In this report, we showed the importance of N-glycosylation for constitutive protein secretions, especially using non-polarized cells. (C) 2009 Elsevier Inc. All rights reserved