Properties of squid axon membrane as revealed by a hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate.
Properties of squid axon membrane as revealed by a hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate.
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疏水探针 2-对甲苯胺基萘-6-磺酸盐揭示了鱿鱼轴突膜的特性。
DOI：
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发表时间：
1971
影响因子：
11.1
通讯作者：
M. Hallett
中科院分区：
文献类型：
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作者：
I. Tasaki;A. Watanabe;M. Hallett
将疏水探针2-对甲苯胺基萘-6-磺酸（TNS）引入鱿鱼巨轴突内部，并在偏振紫外光照射下检测TNS染色轴突的荧光性质。当轴突被电刺激时，观察到荧光的瞬时减少。发现导致这种荧光变化的光波几乎完全偏振，这表明膜中的探针分子是高度取向的。这些TNS染色的轴突的发射光谱进行了检查，在休息和神经兴奋。所获得的结果被认为是支持这样的观点，即神经兴奋的过程是伴随着轴突膜从疏水性到亲水性状态的过渡。
A hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS), was introduced into the interior of a squid giant axon and fluorescene properties of the TNS-stained axon were examined under illumination with polarized ultraviolet light. A transient reduction in fluorescence was observed when the axon was stimulated electrically. The light waves contributing to this fluorescence change were found to be nearly completely polarized, indicating that the probe molecules in the membrane are highly oriented. The emission spectra of these TNS-stained axons were examined at rest and during nerve excitation. The results obtained are considered to support the view that the process of nerve excitation is accompanied by a transition of the axon membrane from a hydrophobic to a hydrophilic state.