RNA-Seq analysis and de novo transcriptome assembly of Cry toxin susceptible and tolerant Achaea janata larvae
RNA-Seq analysis and de novo transcriptome assembly of Cry toxin susceptible and tolerant Achaea janata larvae
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DOI：
10.1038/s41597-019-0160-0
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发表时间：
2019-08-22
期刊：
影响因子：
9.8
通讯作者：
Dutta-Gupta, Aparna
中科院分区：
文献类型：
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作者：
Dhania, Narender K.;Chauhan, Vinod K.;Dutta-Gupta, Aparna
大多数鳞翅目昆虫的幼虫是世界上已知的贪婪的饲养者和重要的农业害虫。摘要印度蓖麻蚜是一种严重危害蓖麻的害虫，造成了巨大的经济损失。基于苏云金芽孢杆菌（Bt）的晶体（Cry）毒素的微生物杀虫剂已经对害虫有效。过度和不加区别地使用Bt基生物农药可能适得其反，并使易感幼虫最终产生抗药性。此外，缺乏足够的基因组和转录组信息的害虫限制了我们的能力，以确定在Bt暴露的敏感和耐受昆虫品系的生理反应改变的分子机制。为了促进害虫物种的生物学、生物化学和分子研究，从而能够更有效地进行生物防治，我们报告了在适当的年龄匹配和饥饿对照下，暴露于Cry毒素的易感性和Cry毒素耐受性的Achaea janata幼虫的中肠从头转录组组装和聚类。
Larvae of most lepidopteran insect species are known to be voracious feeders and important agricultural pests throughout the world. Achaea janata larvae cause serious damage to Ricinus communis (Castor) in India resulting in significant economic losses. Microbial insecticides based on crystalline (Cry) toxins of Bacillus thuringiensis (Bt) have been effective against the pest. Excessive and indiscriminate use of Bt-based biopesticides could be counter-productive and allow susceptible larvae to eventually develop resistance. Further, lack of adequate genome and transcriptome information for the pest limit our ability to determine the molecular mechanisms of altered physiological responses in Bt-exposed susceptible and tolerant insect strains. In order to facilitate biological, biochemical and molecular research of the pest species that would enable more efficient biocontrol, we report the midgut de novo transcriptome assembly and clustering of susceptible Cry toxin-exposed and Cry toxin tolerant Achaea janata larvae with appropriate age-matched and starvation controls.