One Step Purification of Piperine Directly from Piper nigrum L. by High Performance Centrifugal Partition Chromatography
One Step Purification of Piperine Directly from Piper nigrum L. by High Performance Centrifugal Partition Chromatography
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DOI：
10.1080/01496390902775877
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Shen, Min He
中科院分区：
文献类型：
--
作者：
Chen, Zhe;Wu, Jian Bing;Shen, Min He
本研究以正己烷-乙酸乙酯-甲醇-水为两相溶剂体系，成功建立了从胡椒乙醇提取物中直接分离纯化生物活性成分胡椒碱的制备型高效离心分配色谱（HPCPC）方法。使用正己烷-乙酸乙酯-甲醇-水(6:5:6:5, v/v)的上相作为CPC的固定相。在最佳条件下，在一次 CPC 分离中，从 300 mg 粗提物中得到 40 mg 胡椒碱，经 HPLC 测定，纯度为 98.5%。 CPC的峰部分通过(1)H NMR和(13)C NMR鉴定。
In this study, a preparative high performance centrifugal partition chromatography (HPCPC) method for isolation and purification of the bioactive component piperine directly from the ethanol extract of Piper nigrum L. was successfully established by using n-hexane-ethyl acetate-methanol-water as the two-phase solvent system. The upper phase of n-hexane-ethyl acetate-methanol-water (6:5:6:5, v/v) was used as the stationary phase of CPC. Under the optimum conditions, 40 mg of piperine at 98.5% purity, as determined by HPLC, was yielded from 300 mg of the crude extract in a single CPC separation. The peak fraction of CPC was identified by (1)H NMR and (13)C NMR.