Parasitic growth of Pseudomonas aeruginosa in co-culture with the chitinolytic bacterium Aeromonas hydrophila
Parasitic growth of Pseudomonas aeruginosa in co-culture with the chitinolytic bacterium Aeromonas hydrophila
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DOI：
10.1111/j.1462-2920.2010.02271.x
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Philipp, Bodo
中科院分区：
文献类型：
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作者：
Jagmann, Nina;Brachvogel, Hans-Philipp;Philipp, Bodo
降解聚合物的细菌面临着机会性细菌的利用，机会性细菌与降解产物一起生长，而不将能量投入到胞外水解酶的生产中。以甲壳素为碳源、氮源和能源，对铜绿假单胞菌和气单胞菌的共培养进行了研究。在单一培养中，A.铜绿假单胞菌不能与甲壳素共培养，而铜绿假单胞菌能与甲壳素共培养。与两种菌株的共培养物具有双相过程。在第一阶段，铜绿假单胞菌沿着A.第二阶段由A.你好这两个过程是一致的，并依赖于群体感应调节铜绿假单胞菌的次级代谢产物的生产。其中氧化还原活性的吩嗪类化合物绿脓菌素引起了A.通过抑制乌头酸酶阻断柠檬酸循环。因此，A.在共培养的第二阶段，铜绿假单胞菌被迫不完全氧化几丁质，乙酸盐作为最终产物，这支持铜绿假单胞菌的大量生长。总之，铜绿假单胞菌可以通过影响A.以寄生的方式传播。
P>Polymer-degrading bacteria face exploitation by opportunistic bacteria that grow with the degradation products without investing energy into production of extracellular hydrolytic enzymes. This scenario was investigated with a co-culture of Aeromonas hydrophila and Pseudomonas aeruginosa with chitin as carbon, nitrogen and energy source. In single cultures, A. hydrophila could grow with chitin, while P. aeruginosa could not. Co-cultures with both strains had a biphasic course. In the first phase, P. aeruginosa grew along with A. hydrophila without affecting it. The second phase was initiated by a rapid inactivation of and a massive acetate release by A. hydrophila. Both processes coincided and were dependent on quorum sensing-regulated production of secondary metabolites by P. aeruginosa. Among these the redox-active phenazine compound pyocyanin caused the release of acetate by A. hydrophila by blocking the citric acid cycle through inhibition of aconitase. Thus, A. hydrophila was forced into an incomplete oxidation of chitin with acetate as end-product, which supported substantial growth of P. aeruginosa in the second phase of the co-culture. In conclusion, P. aeruginosa could profit from a substrate that was originally not bioavailable to it by influencing the metabolism and viability of A. hydrophila in a parasitic way.