Transforming growth factor beta-1 stimulates articular chondrocyte elaboration of matrix vesicles capable of greater calcium pyrophosphate precipitation
Transforming growth factor beta-1 stimulates articular chondrocyte elaboration of matrix vesicles capable of greater calcium pyrophosphate precipitation
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DOI：
10.1053/joca.2000.0375
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发表时间：
2001-04-01
影响因子：
7
通讯作者：
Rosenthal, AK
中科院分区：
文献类型：
--
作者：
Derfus, BA;Camacho, NP;Rosenthal, AK
目的：通过测定转化生长因子β1（TGFβ）对关节软骨细胞基质囊泡（MV）形成、无机焦磷酸盐（PPi）生成酶核苷三磷酸焦磷酸水解酶（NTPPPH）的比活性和生物矿化能力的影响，确定转化生长因子β1（TGFβ）在早期焦磷酸钙形成中的作用。方法：比较从成熟猪软骨细胞单层+/-TGFβ制备的MV的蛋白质含量， NTPPPH 活性和 ATP 依赖性生物矿化。通过辐射测定和傅里叶变换红外光谱 (FTIR) 测定 MV 引起的焦磷酸钙矿物相的沉淀。结果：与对照单层的 MV 相比，暴露于 TGF β 的单层的 MV 富含 NTPPPH 活性 (P
Objective: To determine the role of transforming growth factor beta1 (TGF beta) in early calcium pyrophosphate formation by measuring its effects on articular chondrocyte matrix Vesicle (MV) formation, specific activity of the inorganic pyrophosphate(PPi)-generating enzyme nucleoside triphosphate pyrophospho-hydrolase (NTPPPH) and biomineralization capacity.Methods: MV elaborated from mature porcine chondrocyte monolayers +/- TGF beta were compared for protein content, NTPPPH activity, and ATP-dependent biomineralization. Precipitation of calcium pyrophosphate mineral phases by MV was determined by a radiometric assay and by Fourier transform infrared spectroscopy (FTIR).Results: MV from monolayers exposed to TGF beta were enriched in NTPPPH activity compared to MV from control monolayers (P