Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis
Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis
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DOI：
10.1038/ki.2011.80
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发表时间：
2011-08-01
影响因子：
19.6
通讯作者：
Chen, Wen-Chi
中科院分区：
文献类型：
--
作者：
Chen, Yung-Hsiang;Liu, Hsin-Ping;Chen, Wen-Chi
几种动物物种被用来研究草酸钙尿石症，然而，一个理想的模型尚未确定。我们使用果蝇作为模式生物，并喂养果蝇致石剂，如乙二醇，羟基-L-脯氨酸和草酸钠。在不同的时间，马氏管，肾脏相当于昆虫，被解剖和偏振光显微镜用来突出双折射晶体。扫描电子显微镜和能量色散X射线光谱证实，晶体成分主要是草酸钙。此外，施用柠檬酸钾成功地减少了乙二醇诱导的晶体的数量并调节了其完整性。因此，果蝇生物矿化模型通过许多致石剂在泌尿系统中产生晶体，允许观察晶体形成，并且适合于遗传操作。该模型可以模拟草酸钙结石形成的病因和临床表现，并有助于鉴定这种疾病的遗传基础。Kidney International（2011）80，369-377; doi：10.1038/ki.2011.80; 2011年3月30日在线发表
Several animal species are used to study calcium oxalate urolithiasis; however, an ideal model has yet to be identified. We used Drosophila as a model organism and fed the flies lithogenic agents such as ethylene glycol, hydroxyl-L-proline, and sodium oxalate. At different times, the Malpighian tubules, the kidney equivalent of insects, were dissected and a polarized light microscope used to highlight the birefringent crystals. Scanning electron microscopy and energy-dispersive X-ray spectroscopy confirmed that the crystal composition was predominately calcium oxalate. Furthermore, administration of potassium citrate successfully reduced the quantity of and modulated the integrity of the ethylene glycol-induced crystals. Thus, the Drosophila model of bio-mineralization produces crystals in the urinary system through many lithogenic agents, permits observation of crystal formation, and is amenable to genetic manipulation. This model may mimic the etiology and clinical manifestations of calcium oxalate stone formation and aid in identification of the genetic basis of this disease. Kidney International (2011) 80, 369-377; doi:10.1038/ki.2011.80; published online 30 March 2011