Preparation and characterization of radioactive castanospermine.
Preparation and characterization of radioactive castanospermine.
复制标题
放射性栗精胺的制备和表征。
DOI：
10.1016/0003-2697(87)90230-2
复制
发表时间：
1987
影响因子：
2.9
通讯作者：
Elbein,AD
中科院分区：
文献类型：
--
作者：
Keenan,RW;Pan,YT;Elbein,AD
描述了氚化粟精胺的制备程序。氚化生物碱在色谱分析上与天然材料相同，并表现出相同的抑制特性。放射性标记的粟精胺与纯化的肠道蔗糖酶紧密结合。凝胶色谱后，每摩尔酶显示结合 1 摩尔放射性生物碱。培养的 MDCK 细胞也显示出吸收标记的粟精胺。该化合物应该是研究负责糖蛋白寡糖加工的酶的有用工具。
A procedure for the preparation of tritiated castanospermine is described. The tritiated alkaloid was shown to be chromatographically identical to the native material and exhibited the same inhibitory properties. Radiolabeled castanospermine tightly bound to purified intestinal sucrase. Following gel chromatography, each mole of enzyme was shown to have bound 1 mol of the radioactive alkaloid. Cultured MDCK cells were also shown to take up the labeled castanospermine. This compound should be a useful tool in the investigation of enzymes that are responsible for the processing of glycoprotein oligosaccharides.