Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde
Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde
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DOI：
10.1002/em.20117
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发表时间：
2005-06-01
影响因子：
2.8
通讯作者：
Lloyd, RS
中科院分区：
文献类型：
--
作者：
Fernandes, PH;Kanuri, M;Lloyd, RS
氯乙烯和巴豆醛是已知的诱变剂和致癌物，通过与DNA反应，形成特异性脱氧鸟苷加合物。为了研究可能的脱氧鸟苷病变的一个子集的致突变潜力，合成氯乙烯和巴豆醛的位点特异性加合物，插入穿梭载体中，并在哺乳动物细胞中复制。氯乙烯和巴豆醛的DNA加合物的突变率分别为2%和5- 6%，因此表明这些加合物可能导致与暴露于这些化学品相关的总体遗传毒性和致癌性。(c)2005 Wiley-Liss，Inc.
Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals. (c) 2005 Wiley-Liss, Inc.