Rab11 is associated with transferrin-containing recycling compartments in K562 cells
Rab11 is associated with transferrin-containing recycling compartments in K562 cells
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DOI：
10.1006/bbrc.1997.7520
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发表时间：
1997-10-20
影响因子：
3.1
通讯作者：
WesslingResnick, M
中科院分区：
文献类型：
--
作者：
Green, EG;Ramm, E;WesslingResnick, M
用3‘RACE聚合酶链式反应检测K562细胞系合成的Rab转录本，在鉴定的GTP结合蛋白中发现了Rab11，这一结果是出乎意料的，因为此前已经发现Rab11与极化和分泌细胞有关，在K562细胞中Rab11的mRNA含量比其他RAB丰富，蛋白水平占总膜蛋白的0.05-0.1%。利用共聚焦免疫荧光显微镜对Rab11进行定位，发现在循环和/或胞外室中，Rab11与转铁蛋白有广泛的重叠，提示Rab11在非极化和非分泌细胞中可能在内化蛋白的运输和再循环中发挥作用。(C)1997年学术出版社。
3'RACE PCR was used La survey Rab transcripts synthesized by the human hematopoietic K562 cell line, Among the identified GTP-binding proteins, Rab11 was discovered, This result was unexpected since Rab11 previously had been found associated with polarized and secretary cells, Rab11 mRNA was abundant compared to that for other Rabs in K562 cells; protein levels represented 0.05-0.1% of total membrane protein. Localization of Rab11 using confocal immunofluoresence microscopy revealed extensive overlap with transferrin in recycling and/or exocytic compartments and suggests that Rab11 in non-polarized and non-secretory cells may play a role ina the trafficking and recycling of internalized proteins. (C) 1997 Academic Press.