Mahuang Xixin Fuzi decoction ameliorates apoptosis via the mitochondrial-mediated signaling pathway in MCM cells
Mahuang Xixin Fuzi decoction ameliorates apoptosis via the mitochondrial-mediated signaling pathway in MCM cells
复制标题
麻黄细辛附子汤通过线粒体介导的信号通路改善MCM细胞凋亡
DOI：
10.1016/j.jep.2022.115538
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发表时间：
2022
影响因子：
5.4
通讯作者：
Rong Rong
中科院分区：
文献类型：
--
作者：
Jia Yang;Qihui Sun;M A Qingyun;Qinhui Yu;Xiaoyun Liu;Yanliang Liu;Yuxiu Han;Yong Yang;Rong Rong
麻黄细辛附子汤作为中医经典方剂，用于治疗老年人发热、鼻塞、头痛等症已有近千年的历史。本研究的目的是评价MXF对热刺激诱导的小鼠心肌细胞（MCM）细胞凋亡的影响及其可能的机制。MCM细胞模型的细胞凋亡通过PCR计算的温度控制系统以43 °C下的逐渐加热模式诱导1小时。使用实时细胞分析仪（RTCA）技术测定细胞毒性效应。Annexin V-FITC/7-AAD染色和JC-1荧光用于评估凋亡。特异性底物，酶联免疫吸附试验（ELISA），和蛋白质印迹法被用来确定蛋白质在尿道介导的途径。采用超高效液相色谱-电喷雾串联质谱法对小鼠心脏中的化学成分进行了鉴定。MXF通过神经元介导的信号通路抑制细胞凋亡，包括改善MMP减少，阻断线粒体Cyt C释放，降低Bax水平和增加Bcl-2水平，抑制细胞质组分中的caspase-9和caspase-3活化。此外，MXF中作用于心脏的成分主要为乌头类生物碱和乌头碱类生物碱。结果表明，MXF处理显着减少MCM细胞凋亡诱导的热刺激，这可能归因于细胞介导的信号通路。·首次证实名方MXF煎剂能有效保护热刺激诱导的MCM细胞凋亡。·细胞凋亡可以通过调节线粒体功能和细胞凋亡途径来预防。·这项研究确定了与细胞凋亡相关的心脏疾病的潜在治疗策略。
Mahuang Xixin Fuzi Decoction (MXF), as a classical prescription of traditional Chinese medicine (TCM), has been used to treat the symptoms of fever, nasal congestion and headache in elderly people for almost a thousand years. The purpose of this study was to evaluate the effects and possible mechanisms of MXF on thermal stimulation-induced mouse cardiac myocytes (MCM) cell apoptosis. The apoptosis of the MCM cell model was induced by a PCR-calculated temperature control system with a gradual heating pattern at 43 °C for one hour. The cytotoxic effects were determined using real-time cell analyzer (RTCA) technology. Annexin V-FITC/7-AAD staining, and JC-1 fluorescence were used to assess apoptosis. Specific substrates, enzyme-linked immunosorbent assays (ELISAs), and Western blotting were used to identify proteins in the mitochondrial-mediated pathway. The identification of chemical components in the mouse heart was performed by ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry analysis. MXF inhibited apoptosis through the mitochondrial-mediated signaling pathway, including ameliorating MMP reduction, blocking mitochondrial Cyt C release, reducing Bax levels and increasing Bcl-2 levels, suppressing caspase-9 and caspase-3 activation in cytoplasmic fractions. Moreover, the components of MXF that act on the heart are mainly ephedra alkaloids and aconitine alkaloids. The findings demonstrated that MXF treatment markedly reduced MCM cell apoptosis induced by thermal stimulation, which may be ascribed to the mitochondrial-mediated signaling pathway. • It was first confirmed that the famous formula MXF decoction could effectively protect MCM cell apoptosis induced by thermal stimulation. • Apoptosis may be prevented via modulation of mitochondrial functions and apoptotic pathways. • This study identifies a potential therapeutic strategy for cardiac diseases associated with apoptosis.