Functional characterization of class I SlHSP17.7 gene responsible for tomato cold-stress tolerance
Functional characterization of class I SlHSP17.7 gene responsible for tomato cold-stress tolerance
复制标题
负责番茄冷胁迫耐受性的 I 类 SlHSP17.7 基因的功能特征
DOI：
10.1016/j.plantsci.2020.110568
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Jiang Jing
中科院分区：
文献类型：
--
作者：
Zhang Ning;Zhao Huaiyin;Shi Jiewei;Wu Yuanyuan;Jiang Jing
Small heat shock proteins (sHSPs) increase stress tolerance in a wide variety of organisms and enable them to endure changes in their environment. However, the molecular mechanism by which sHSPs protect plants against cold stress is unknown. Here, the sHSP of tomato namedSlHSP17.7(Solyc06g076540.1.1) has the characteristic of low temperature induced expression in BL21(DE3)E. coliand a molecular chaperone functionin vitro. Overexpression of SlHSP17.7 showed a tolerant response to cold stress treatment due to an induce intracellular sucrose and less accumulation of ROS. Yeast two-hybrid assays showed that SlHSP17.7 is a binding partner of the cation/Ca2+exchanger (SlCCX1-like; Solyc07g006370.1.1). This interaction was confirmed by pull down and bimolecular fluorescence complementation (BiFC) assays. HighSlHSP17.7and lowSlCCX1-likelevels alleviated programed cell death (PCD) under cold stress. Thus, SlHSP17.7 might be a cofactor ofSlCCX1-liketargeting endoplasmic reticulum (ER) membrane proteins, retaining intracellular Ca2+homeostasis, and decreasing cold stress sensitivity. These findings provide a sound basis for genetic engineering of cold stress tolerance in tomato.
