Gene silencing of translationally controlled tumor protein (TCTP) by siRNA inhibits cell growth and induces apoptosis of human prostate cancer cells
Gene silencing of translationally controlled tumor protein (TCTP) by siRNA inhibits cell growth and induces apoptosis of human prostate cancer cells
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DOI：
10.3892/ijo_00000252
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发表时间：
2009-05-01
影响因子：
5.2
通讯作者：
Ramaswamy, Kalyanasundaram
中科院分区：
文献类型：
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作者：
Gnanasekar, Munirathinam;Thirugnanam, Sivasakthivel;Ramaswamy, Kalyanasundaram
翻译控制肿瘤蛋白(TCTP)是一种新型的抗细胞凋亡蛋白，在前列腺癌等多种肿瘤细胞中高表达。然而，研究TCTP在前列腺癌中的作用的研究很少。因此，在本研究中，我们评估了基于小干扰RNA(SiRNA)的TCTP基因在前列腺癌细胞中的敲除效果。检测细胞增殖和凋亡情况。我们的结果表明，与正常前列腺上皮细胞相比，TCTP在LNCaP细胞中高表达。TCTP siRNA可显著降低LNCaP细胞TCTP的mRNA和蛋白表达水平。TCTP表达降低与LNCaP细胞存活率下降有关。进一步的分析表明，LNCaP细胞通过caspase-8和caspase-3依赖的途径发生凋亡。本研究结果提示，通过siRNA方法靶向TCTP基因治疗前列腺癌具有潜在的应用前景。
Translationally controlled tumor protein (TCTP) is a novel anti apoptotic protein which is highly expressed in several cancer cell types including prostate cancer. However, studies investigating the role of TCTP in prostate cancer are scarce. Therefore, in this study we evaluated the effect of small interference RNA (siRNA) based knocking down of TCTP gene in prostate cancer cells. Cell proliferation and apoptosis were evaluated. Our results showed that TCTP is highly expressed in LNCaP cells compared to normal prostate epithelial cells. Transfection with TCTP siRNA specifically and drastically reduced the expression of both mRNA and protein levels of TCTP in LNCaP cells. The decreased expression of TCTP was associated with decreased viability of LNCaP cells. Further analysis of the transfected LNCaP cells showed that they undergo apoptosis via caspase-8 and caspase-3 dependent pathways. Results presented herein suggest a potential therapeutic application for prostate cancer by targeting TCTP gene using an siRNA approach.