RECENT DEVELOPMENTS IN HETEROLOGOUS PROTEIN-PRODUCTION IN ESCHERICHIA-COLI
RECENT DEVELOPMENTS IN HETEROLOGOUS PROTEIN-PRODUCTION IN ESCHERICHIA-COLI
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DOI：
10.1016/0167-7799(94)90021-3
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发表时间：
1994-11-01
影响因子：
17.3
通讯作者：
HOCKNEY, RC
中科院分区：
文献类型：
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作者：
HOCKNEY, RC
在过去的三到四年里，我们在蛋白质折叠、蛋白质跨生物膜转运以及分子伴侣在这些过程中的作用方面取得了显着的进展。与大肠杆菌表达系统的最新发展相结合，这种理解导致了以可溶形式积累蛋白质、从细胞质分泌蛋白质、在细胞质膜中积累蛋白质以及将蛋白质引导至细胞外膜用于表面展示的能力的提高。这些进展表明E.现在应该认真考虑大肠杆菌的应用，仅仅几年前，人们还认为它超出了这种生物的范围。
During the past three to four years, remarkable progress has been made in our understanding of protein folding, protein translocation across biological membranes, and the role of molecular chaperones in these processes. In conjunction with recent developments in Escherichia coli expression systems, this understanding has led to an improved capability to accumulate proteins in a soluble form, secrete proteins from the cell cytoplasm, accumulate proteins in the cytoplasmic membrane, and direct proteins to the outer membrane of the cell for surface display. These advances suggest that E. coli should now be considered seriously for applications that, only a few years ago, would have been thought beyond the scope of this organism.