THE ELONGATION AND ORIENTATION OF CULTURED ENDOTHELIAL-CELLS IN RESPONSE TO SHEAR-STRESS
THE ELONGATION AND ORIENTATION OF CULTURED ENDOTHELIAL-CELLS IN RESPONSE TO SHEAR-STRESS
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DOI：
10.1115/1.3138567
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发表时间：
1985-01-01
影响因子：
1.7
通讯作者：
NEREM, RM
中科院分区：
文献类型：
--
作者：
LEVESQUE, MJ;NEREM, RM
血管内皮细胞与动脉壁附近的血流排列一致，在高剪切区呈椭球状，在低剪切区呈多角形。为了定量研究这种反应的性质，在Thermanox塑料盖上生长的牛主动脉内皮细胞在平行板流室中暴露于10、30和85达因/cm2的剪切应力水平长达24小时。利用计算机分析系统量化细胞的伸长程度与细胞取向角和时间的变化有关。结果表明：(1)在剪切应力的影响下内皮细胞随流动方向定向，(2)细胞随流动方向排列所需的时间比细胞伸长所需的时间稍长，(3)排列程度与内皮细胞形状之间存在很强的相关性，(4)内皮细胞在较高的剪切应力下会变得更细长。
Vascular endothelial cells appear to be aligned with the flow in the immediate vicinity of the arterial wall and have a shape which is more ellipsoidal in regions of high shear and more polygonal in regions of low shear stress. In order to study quantitatively the nature of this response, bovine aortic endothelial cells grown on Thermanox plastic coverslips were exposed to shear stress levels of 10, 30, and 85 dynes/cm2 for periods up to 24 hr using a parallel plate flow chamber. A computerbased analysis system was used to quantify the degree of cell elongation with respect to the change in cell angle of orientation and with time. The results show that (i) endothelial cells orient with the flow direction under the influence of shear stress, (ii) the time required for cell alignment with flow direction is somewhat longer than that required for cell elongation, (iii) there is a strong correlation between the degree of alignment and endothelial cell shape, and (iv) endothelial cells become more elongated when exposed to higher shear stresses.