Autologous cultured mucosal graft to cover large intraoral mucosal defects: a clinical study.
Autologous cultured mucosal graft to cover large intraoral mucosal defects: a clinical study.
复制标题
自体培养粘膜移植物覆盖大面积口腔内粘膜缺损：一项临床研究。
DOI：
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发表时间：
2003
影响因子：
1.9
通讯作者：
N. Grossman
中科院分区：
文献类型：
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作者：
L. Bodner;N. Grossman
目的
这项研究评估了培养的粘膜移植物(CMG)修复手术切除黏膜病变引起的口腔内大型粘膜缺损的性能。
患者和方法
11例患者(男性10例，女性1例；平均年龄52.4±14.1岁)接受了黏膜缺损后CMG治疗。手术前几周，取自临床健康口腔粘膜的活检标本(0.2-0.5厘米)。黏膜上皮细胞在体外培养，覆盖成纤维细胞饲养层。通常，在3-4周内，会产生多层薄片(50-250 cm(2))。将培养的薄片放置在黏膜缺损处(48.4+/-21.7 cm(2)；8~70 cm(2))，用缝线固定在邻近组织上。
结果
术后一周，CMG成活并附着在创面床上。术后3周，移植部位光滑，角化，无感染或瘢痕收缩。术后3个月，移植区被健康的粘膜覆盖，与邻近的粘膜难以区分。
结论
CMG是修复大型口腔粘膜缺损的有效移植材料。
PURPOSE
The study evaluated the performance of cultured mucosal grafts (CMG) for large intraoral mucosal defects caused by surgical excision of mucosal pathology.
PATIENTS AND METHODS
Eleven patients (10 men and 1 woman; mean age, 52.4 +/- 14.1 years) were treated using CMG following mucosal defects. A biopsy specimen (0.2 to 0.5 cm(2)) was taken from a clinically healthy oral mucosa a few weeks before the surgery. Mucosal epithelial cells were cultured in vitro over a feeder layer of fibroblasts. Usually, within 3 to 4 weeks, multilayered sheets (50 to 250 cm(2)) were generated. The cultured sheets were placed on the mucosal defects (48.4 +/- 21.7 cm(2); 8 to 70 cm(2)) and anchored to the adjacent tissue with sutures.
RESULTS
One week after surgery, the CMG survived and adhered to the wound bed. Three weeks postoperatively, the grafted site was smooth and keratinized, without infection or scar contraction. Three months postoperatively, the grafted area was covered with a healthy mucosa, indistinguishable from the adjacent mucosa.
CONCLUSION
CMG is a useful grafting material for large intraoral mucosal defects.