Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance investigation of deuterium-labelled N-hexadeconoylgalactosylceramides (cerebrosides).
Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance investigation of deuterium-labelled N-hexadeconoylgalactosylceramides (cerebrosides).
复制标题
细胞表面和细胞膜结构的物理研究。
DOI：
10.1016/0005-2736(79)90043-9
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
E. Oldfield
中科院分区：
文献类型：
--
作者：
R. Skarjune;E. Oldfield
1.1.用自制的超导磁共振波谱仪，在34.1MHz的频率下，用自旋回波技术，获得了N-棕榈酰半乳糖神经酰胺（β-半乳糖苷）的氘傅里叶变换核磁共振谱。研究了温度和胆固醇对氘光谱的影响。结果表明，当在相同的降低温度下比较时，棕榈酰半乳糖基神经酰胺的液晶相中的烃链组织与在类似链长的甘油磷脂中所见的基本相同。特别是，在2′-标记的N-棕榈酰半乳糖神经酰胺中观察到两组四极分裂，表明与先前在磷脂中注意到的不等价的氘核。3.3.观察到头基C-6-标记的N-棕榈酰半乳糖神经酰胺的两组四极分裂。有人提出这些信号来自对映体RandSlipids，并且羟甲基的运动是缓慢的（大于10−5s）。这些结果表明极性头基区域存在氢键网络。
1.1. Deuterium Fourier transform nuclear magnetic resonance spectra of a series ofN-palmitoylgalactosylceramides (cerebrosides) specifically labelled with deuterium at one of positions 2′, 6′, 10′ and 16′ of the acyl chain, or in the C-6 hydroxymethyl group of the galactose residue, have been obtained using a spin-echo technique at 34.1 MHz with a homebuilt superconducting magnet spectrometer.2.2. The effects of temperature and cholesterol on the deuterium spectra have been investigated. The results indicate, when compared at the same reduced temperature, that the hydrocarbon chain organization in the liquid crystalline phase of palmitoylgalactosylceramide is essentially identical to that seen in similar chain length glycerophospholipids. In particular, two sets of quadrupole splittings are seen for a 2′-labelledN-palmitoylgalactosylceramide, indicating non-equivalent deuterons as noted previously for phospholipids.3.3. Two sets of quadrupole splittings are observed for the headgroup C-6-labelledN-palmitoylgalactosylceramide. It is proposed that these signals arise from the enantiomericRandSlipids, and that motion of the hydroxymethyl group is slow (greater than 10−5s). These results suggest the presence of a hydrogen bond network in the polar headgroup region.