Bioactive components and antibacterial activities of hydrolate extracts by optimization conditions from Paeonia ostii T. Hong ＆ J. X. Zhang
Bioactive components and antibacterial activities of hydrolate extracts by optimization conditions from Paeonia ostii T. Hong ＆ J. X. Zhang
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DOI：
10.1016/j.indcrop.2022.115737
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发表时间：
2022-11
影响因子：
5.9
通讯作者：
Ziwei Xin;Weizong Yang;Yihao Duan;Wenjia Wang;L. Niu;Daoyang Sun;Yanlong Zhang
中科院分区：
文献类型：
--
作者：
Ziwei Xin;Weizong Yang;Yihao Duan;Wenjia Wang;L. Niu;Daoyang Sun;Yanlong Zhang
牡丹是一种重要的经济植物，我国分布有九种野生植物。其中，牡丹。 Hong & J. X. Zhang 因其观赏、药用和食用油价值而成为栽培最多的野生物种之一。然而，P. 水合物提取物的生化成分和生物活性。 ostii 很大程度上不为人所知。在这项研究中，我们研究了各种 P 的潜力。用作抗菌剂的器官。通过单因素优化和Box-Behnken设计（BBD）实验确定最佳水溶胶提取条件为：提取时间4 h，温度90 ℃，压力0.35 MPa，比例6:1。总共从 P. 中鉴定出 150 种挥发性化学物质并对其进行了定量。 ostii通过气相色谱-质谱法（GC-MS）。检测了对八种革兰氏阳性和革兰氏阴性细菌的抗菌活性。挥发性物质中，单萜类化合物（31.36%~77.28%）含量最高，其次是醇类（17.69%~26.82%）。定量测试证明了P。 ostiiHydrsols 对金黄色葡萄球菌和溶血链球菌-β 显着有效。特别是，新生长的叶子纯露比其他器官的纯露表现出更明显的抗菌作用，抑菌区和最低抑菌浓度 (MIC) 分别为 10.65–17.5 mm 和 0.78–12.5 mg/mL。结果表明，水合物是从P. ostii对一些常见的皮肤感染和食源性细菌病原体具有显着的抗菌活性，这表明P. ostii可能是食品、药品和化妆品抗菌剂的潜在来源。
Tree peony is an economically important plant with nine wild species distributed in China. Among them,Paeonia ostiiT. Hong & J. X. Zhang is one of the most cultivated wild species for its ornamental, medicinal, and edible oil values. However, the biochemical composition and biological activities of hydrolate extracts fromP. ostiiare largely unknown. In this study, we investigated the potentials of variousP. ostiiorgans for being used as antibacterial agents. The single-factor optimization and Box-Behnken Design (BBD) experiments were performed to determine the optimal hydrosol extraction conditions as follows: extraction duration of 4 h, temperature of 90 °C, pressure of 0.35 MPa, and ratio of 6:1. In total, 150 volatile chemicals were identified and quantified fromP. ostiithrough gas chromatography-mass spectrometry (GC-MS). The antibacterial activities against eight Gram-positive and Gram-negative bacterial were examined. Among the volatile substances, mono-terpenoids (31.36–77.28 %) was of the highest content inP.ostii, followed by alcohols (17.69–26.82 %). The quantitative test demonstrated theP. ostiihydrosols being significantly effective againstStaphylococcus aureusandStreptococcus hemolytis-β. In particular, the newly-grown leaf hydrosols displayed more pronounced antibacterial effects than those from other organ, with the inhibition zones and minimal inhibitory concentrations (MICs) being 10.65–17.5 mm and 0.78–12.5 mg/mL, respectively. The results indicate that the hydrolate extracts fromP. ostiipossessed remarkable antibacterial activities against some common skin-infecting and food-borne bacterial pathogens, implying thatP. ostiicould be a potential source for antibacterial agents for food, pharmaceutical, and cosmetics.