An LC method for the analysis of phosphatidylcholine hydrolysis products and its application to the monitoring of the acyl migration process
An LC method for the analysis of phosphatidylcholine hydrolysis products and its application to the monitoring of the acyl migration process
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DOI：
10.1016/j.talanta.2012.01.018
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发表时间：
2012-05-30
期刊：
影响因子：
6.1
通讯作者：
Wawrzenczyk, Czeslaw
中科院分区：
文献类型：
--
作者：
Kielbowicz, Grzegorz;Smuga, Damian;Wawrzenczyk, Czeslaw
建立了一种高效液相色谱-电荷气溶胶检测器（CAD）测定磷脂酰胆碱（1，2-二棕榈酰-sn-甘油-3-磷酸胆碱）及其水解产物1-羟基-2-棕榈酰-sn-甘油-3-磷酸胆碱和1-棕榈酰-2-羟基-sn-甘油-3-磷酸胆碱、sn-甘油-3-磷酸胆碱和棕榈酸的方法。在反相/亲水相互作用固定相和由乙腈：甲醇：10 mM乙酸铵溶液组成的移动的相上实现目标化合物的分离。该方法适用于控制LPC区域异构体在PC合成或改性中最常用的溶剂中的酰基迁移过程。(C)2012爱思唯尔有限公司版权所有。
An assay for quantitative analysis of phosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and its hydrolysis products: 1-hydroxy-2-palmitoyl-sn-glycero-3-PhosPhocholine and 1-palmitoyl-2-hydroxy-sn-glycero-3-PhosPhocholine, sn-glycero-3-phosphocholine and palmitic acid using high-performance liquid chromatography with charge aerosol detector (CAD) was developed. The separation of the compounds of interest was achieved on a reversed-phase/hydrophilic interaction stationary phase with a mobile phase consisting of acetonitrile:methanol:10 mM ammonium acetate solution. The method was applied to control the acyl migration process of LPC regioisomers in the most common solvents used in the synthesis or modification of PC. (C) 2012 Elsevier B.V. All rights reserved.